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Insert Specimen
1. Load specimen onto specimen holder
single specimen holder
use tweezers No.1 to open clip
place specimen grid in holder
use same tweezers to clamp clip on specimen
tap holder to ensure specimen is firmly in place
2. Check if the following are nice and clean:
the two O-rings and remove fluff etc
the yellow ceramic ring has a flat face that must be free of grease, swarf and fluff.
Remove debris with lint-free tissue
3. Insert specimen holder into microscope
Carefully place holder into TEM, align guide pin with guide groove, push in gently
without turning until the holder stops
(while still pushing the holder) turn switch from 'Air' to 'Pump', you must pull switch
out and push it up. You should hear the valve opening.
wait for green light to come on
after green light comes on, get a good grip on the specimen holder and rotate
clockwise a few (~10
microscope a short distance (~0.5cm) until it stops (don't let the holder slam into the
next position)
Still with a good grip rotate clockwise several (~80
slowly allow it to go into the microscope (MAINTAIN A TIGHT GRIP AS THE VACUUM
IS VERY POWERFUL, don't let the holder slam into position)
After insertion, tap the end of the holder
4. Select appropriate holder in opened 'Specimen' window.
5. Confirm the HT is at 80.03 kV and is READY. Use 'beam controller' window.
EM Suite 15/11/2016
o
) degrees until it stops and , then allow it to go into the
o
) degrees until holder stops and
P a g e
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