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JEM1400 Transmission Electron Microscope operation
Starting Session
1. Turn on all computer monitors
2. On the JEOL computer, if not logged in, select JEMUser account (password 'JEMUser')
and log in (you might need to use JEOL computer mouse and keyboard at the back of
JEOL monitor). After this, the computers should be controllable by AMT computer mouse
and keyboard. If not, notify EM Suite staff.
3. JEOL computer. If 'TEM center' is not opened, double click 'TEM center domain' icon on
desktop.
4. JEOL computer. Use 'Valve/vacuum monitor' window. Check that the gun and column
vacuum is reasonable - all chambers except 'Specimen chamber' should say 'Evac Ready'.
5. JEOL computer. Use 'beam controller' window. If the 'High tension (HT)' is off, turn it on.
It should be set to 80.03 kV (if a different value is needed ask EM suite staff).
6. Aperture settings. Check it on the microscope column.
condenser aperture should be IN (adjust with lever; left is in, right is out).
objective aperture should be OUT (red dot is out, white dots – in and show relative
size of aperture). The objective aperture may be IN after you know you have a beam
and if you want the extra contrast.
7. Spot size – 2. Use 'TEM System Task Bar' window. To change use 'SPOT SIZE' knob (left
console).
EM Suite 15/11/2016
AMT camera
computer
EM
JEOL computer
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Summary of Contents for JEOL JEM1400
- Page 1 2. On the JEOL computer, if not logged in, select JEMUser account (password ‘JEMUser’) and log in (you might need to use JEOL computer mouse and keyboard at the back of JEOL monitor). After this, the computers should be controllable by AMT computer mouse and keyboard.
- Page 2 Hints or tips 1. ‘BRIGHTNESS’ knob (left console) - CLOCKWISE (CW) – spread beam = decrease illumination - ANTI-CLOCKWISE (ACW) – condense beam = increase illumination 2. CRS button –any controls are in the coarse mode (16-fold change) when the nearby is illuminated 3.
- Page 3 Viewing and Imaging Specimen 1. Insert specimen (see page 5-6 of this instruction) 2. Use ‘Valve/vacuum monitor’ window. Make sure the specimen chamber says ‘Evac ready’. 3. Turn on filament. Use ‘beam controller’ window. Filament is on when ‘On’ and ‘Beam Ready’...
- Page 4 Press ‘Standard Focus’ button on right console to neutralize the focus Press Wobbler button X or Y (‘IMG WOB’) on right console. Use the ‘+Z’ and ‘−Z’ buttons on right console (or ‘+Z’ and ‘−Z’ buttons in the ‘Stage Controller’...
- Page 5 Insert Specimen 1. Load specimen onto specimen holder single specimen holder use tweezers No.1 to open clip place specimen grid in holder use same tweezers to clamp clip on specimen tap holder to ensure specimen is firmly in place 2.
- Page 6 Remove Specimen 1. Turn filament off. Use ‘Beam controller’ window. Click ‘Filament OFF’ button 2. Neutralize the stage. Press ‘Stage Neutral’ button in ‘Stage Controller’ window (stage will move to XYZ center and title angle 0°). Check that in ‘TEM System Task Bar’ window, tab ‘Stage’.
- Page 7 Alignments 1. Use fluorescent screen and, if needed, binoculars with small fluorescent screen 2. Center condenser aperture Set magnification 5,000x using MAG/CAML knob (right console) Condense beam to a spot with ‘BRIGHTNESS’ knob Move beam to center of fluorescent screen with Shift (‘X’, ‘Y’) knobs ...
- Page 8 h) Condense the beam to smallest size i) If beam position will be not in the centre of the screen, press F4 (‘Gun Align’) button on right console and use the SHIFT knobs, X and Y, to move the beam back to the centre.
- Page 9 Set magnification 80,000-100,000x using MAG/CAML knob (right console) Centre the beam, use the SHIFT knobs, X and Y, on the left and right consoles. It may help condense the beam before doing this (BRIGHTNESS knob). use AMT camera capture engine program ...
- Page 10 Record Images with AMT Camera 1. Set Magnification of 4,000x using MAG/CAML knob (right console) 2. Centre the beam, use the SHIFT knobs, X and Y, on the left and right consoles. It may help condense the beam before doing this (BRIGHTNESS knob). 3.
- Page 11 15. DO NOT install any software (including drivers) on the AMT computer. If your drive will not work without installing drivers on the AMT computer, then find another drive that will. 16. DO NOT plug anything into JEOL computer. EM Suite 15/11/2016 P a g e...
- Page 12 Ending Session 1. Set magnification to 4000× (MAG/CAML knob (right console)) 2. Spread beam (BRIGHTNESS knob (left console)) to cover approximately half of the screen, center beam if uncentered (SHIFT knobs, X and Y, on the left and right consoles). 3.
- Page 13 EM will start. Switch on the AMT computer and camera control Switch on the HP computer. Log on using the HP keyboard and JEOL MOUSE at rear of EM. Check for communications. TEM Center loads automatically.
- Page 14 Generate an electron beam Use the Beam controller window HT voltage – ON (may already be on i.e. green) Filament – ON Bias – adjust Bias Coarse Bias Fine to change the beam current. Need 12 – 16µA emission For 80kV, the beam current = 44µA.