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JEOL JEM1400 Operation page 6

Transmission electron microscope

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Remove Specimen
1. Turn filament off. Use 'Beam controller' window. Click 'Filament OFF' button
2. Neutralize the stage. Press 'Stage Neutral' button in 'Stage Controller' window (stage will
move to XYZ center and title angle 0°). Check that in 'TEM System Task Bar' window, tab
'Stage'.
3. Put fluorescent viewing screen down. Remove the cover of the viewing screen and check
the screen position. If needed use 'F1' button to low it down.
4. Remove holder
 pull sample holder straight out until it stops
 turn holder anti-clockwise several (~80
STOP and get help, something is wrong
 pull holder out a short (~5mm) distance until you can turn the holder further a few
o
(~10
) degrees anti-clockwise until it stops
 turn switch from 'Pump' to 'Air', you must pull switch out and push it down
 switch on Nitrogen gas – open butterfly valve on the wall.
 wait for amber and green lights to turn off and hissing sound ( ~10sec).
 close Nitrogen gas – close butterfly valve on the wall.
 gently remove holder out of microscope. Be careful not to touch the holder beyond
rubber O-rings, as this will introduce grease and dirt into the microscope.
5. Remove grid from holder
single specimen holder
 use tweezers No.1 to open clip
 remove grid
EM Suite 15/11/2016
o
) degrees until it stops, if you feel resistance,
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