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Columns manufactured by Showa Denko K.K Japan
Made in Japan
HPLC Columns
MANUAL
IC Y-521
Shodex HPLC Columns
Europe, Middle East, Africa, Russia
For technical support please use
contact details shown below:
SHOWA DENKO EUROPE GmbH
Shodex Business
Konrad-Zuse-Platz 3
81829 Munich, Germany
E-mail:
support@shodex.de
Phone:
+49 (0)89 93 99 62 37
www.shodex.de
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Related Manuals for Showa Denko Shodex IC Y-521
Summary of Contents for Showa Denko Shodex IC Y-521
- Page 1 For technical support please use contact details shown below: SHOWA DENKO EUROPE GmbH Shodex Business Konrad-Zuse-Platz 3 81829 Munich, Germany Columns manufactured by Showa Denko K.K Japan E-mail: support@shodex.de Made in Japan Phone: +49 (0)89 93 99 62 37 www.shodex.de...
- Page 3 (Please read this manual carefully before using the column to ensure performance and life.) 1. Introduction Shodex IC Y-521 is designed for exclusive use in ion chromatography for cationic separation. Packed with a polystyrene-based strong cation exchange resin prepared specially for ion chromatography, the column is suited for use in separation of alkaline metals, alkaline earth metals and amines.
- Page 4 IC Y-521 Ver. 640 Manual 2) For separation of alkaline earth metals A solution of ethylene diamine and tartaric acid is the mobile phase. NOTE : Recipe for mobile phase of 2-mM ethylene diamine/4-mM tartaric acid Dissolve 6.01 g of ethylene diamine and 30.02 g of tartaric acid in ion exchange water that has passed through a 0.45-µm-mesh filter to obtain one liter of the solution.
- Page 5 IC Y-521 Ver. 640 Manual CAUTION! Although aqueous nitric acid can be used with such a chromatograph, the inside surface of the pipes is likely to partially dissolve out until it has been completely passivated. Since the substances so dissolved in the aqueous nitric acid deteriorate the performance of the column, if passed through it, take the following procedure to purge the chromatograph before mounting the column on it.
- Page 6 IC Y-521 Ver. 640 Manual 3) Cap both ends and store the column in a place where no direct sunlight reaches and ambient temperature fluctuates little. CAUTION! i) Do not store the column with aqueous nitric acid or 10 mM or more contained in it. ii) In case the column is to be stored longer than one month, pass aqueous 0.1-M sodium chloride through it at a flow rate of 0.5 ml/min to change the ion exchanger to an Na type and then pass aqueous 0.1% sodium azide before capping both ends.
- Page 7 IC Y-521 Ver. 640 Manual Notes Shodex IC Y-521P is recommended. 10 Shodex IC Y-521L is recommended. Since It Is filled with ion exchange water by the manufacturer, be sure before use to pass ca. 30 ml or the mobile phase through it. The line filter column can be reactivated by passing through it 50 ml each or aqueous 0.5-N sodium hydroxide, 0.1-M tartaric acid and 0.5-N nitric acid in that order.
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