Showa Denko Shodex Asahipak GF-HQ Series Operation Manual

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Operation Manual Shodex Asahipak GF-HQ series
Shodex™ Asahipak GF-HQ series
Europe, Middle East, Africa, Russia
www.shodex.de
Operation Manual
Sales Office:
Showa Denko Europe GmbH
Shodex Business
Konrad-Zuse-Platz 3
81829 Munich
Germany
Version 2106029E
Technical Support:
info@shodex.de
+49 (0)89 / 93 99 62 37
Quotes and Orders:
order@shodex.de
+49 (0)89 / 93 99 62 34

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Summary of Contents for Showa Denko Shodex Asahipak GF-HQ Series

  • Page 1 Operation Manual Shodex Asahipak GF-HQ series Version 2106029E Operation Manual Shodex™ Asahipak GF-HQ series Sales Office: Technical Support: Showa Denko Europe GmbH info@shodex.de Shodex Business +49 (0)89 / 93 99 62 37 Konrad-Zuse-Platz 3 81829 Munich Quotes and Orders: Europe, Middle East, Africa, Russia Germany order@shodex.de...
  • Page 2 Certificate of Analysis (CoA) are matching and correct. Introduction Thank you for purchasing the Shodex™ product. Shodex Asahipak GF-HQ series is a polymer-based size exclusion chromatography column. They work well with both aqueous and organic solvents (multi- solvent column).
  • Page 3 Operation Manual Shodex Asahipak GF-HQ series Version 2106029E Target Molecular Weight Product Code Product Name Exclusion Limit Range F7600000 Asahipak GF-210 HQ 300 – 4,000 9,000 F7600001 Asahipak GF-310 HQ 300 - 30,000 40,000 F7600002 Asahipak GF-510 HQ 5,000 - 200,000...
  • Page 4 Operation Manual Shodex Asahipak GF-HQ series Version 2106029E deterioration from the solvent replacement. It is recommended to dedicate the column for this specific use. (4) Surfactants such as SDS and Brij-35 can also be added to the eluent. However, since surfactants tend to remain on the column, solvent replacement after their use takes a longer lime than replacement from general solvents.
  • Page 5 Operation Manual Shodex Asahipak GF-HQ series Version 2106029E (5) Viscosity of high molecular weight compound is largely influenced by its molecular weight and concentration. Samples with high viscosity cause peak broadening and elution delay, and this makes it difficult to obtain their accurate molecular weight distributions. In general, the larger the molecular weight of the compound, the higher its viscosity becomes.
  • Page 6 Operation Manual Shodex Asahipak GF-HQ series Version 2106029E provide linear calibration curves over a set molecular weight range. If a GF-7M HQ was used together with columns with different pore sizes, this changes the mixing ratio of the gels and consequently it may deform the linear calibration curve.
  • Page 7 Operation Manual Shodex Asahipak GF-HQ series Version 2106029E the washing solution flow from the column outlet go directly into the waste container and not let the solution go through the detector. <Cleaning Method> (1) Insoluble components that block the column inlet may be removed by reversing the flow direction, i.e., introducing the eluent from the column outlet, with flow rate at less than half of the recommended...
  • Page 8 3-3 If the Shodex product is disposed of 3-4 If the Shodex product is resold by the user without giving prior written notice to Showa Denko K.K. 3-5 If the performance of the Shodex product is not conform to the specification of the attached CERTIFICATE OF ANALYSIS due to any of the reasons below: a.

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