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Operation Manual Shodex IEC SP-825
Europe, Middle East, Africa, Russia
www.shodex.de
Operation Manual
Shodex™ IEC SP-825
Sales Office:
Showa Denko Europe GmbH
Shodex Business
Konrad-Zuse-Platz 3
81829 Munich
Germany
Version 211129E
Technical Support:
info@shodex.de
+49 (0)89 / 93 99 62 37
Quotes and Orders:
order@shodex.de
+49 (0)89 / 93 99 62 34
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Summary of Contents for Showa Denko Shodex IEC SP-825
- Page 1 Operation Manual Shodex IEC SP-825 Version 211129E Operation Manual Shodex™ IEC SP-825 Sales Office: Technical Support: Showa Denko Europe GmbH info@shodex.de Shodex Business +49 (0)89 / 93 99 62 37 Konrad-Zuse-Platz 3 81829 Munich Quotes and Orders: Europe, Middle East, Africa, Russia Germany order@shodex.de...
- Page 2 Certificate of Analysis (CoA) are matching and correct. Introduction Thank you for purchasing the Shodex™ product. Shodex IEC SP-825 is a strong cation exchange column filled with hydrophilic polymer-based gel modified with sulfopropyl group. It is an ideal column for analyzing various proteins, peptides, and components of nucleic acids as it has a low adsorption tendency towards hydrophobic compounds.
- Page 3 Operation Manual Shodex IEC SP-825 Version 211129E Usable Conditions Temperature Flow Rate (mL/min) Maximum Pressure Product Name pH Range Range (°C) (MPa) (Per Column) Recommended Maximum 10 - 50 IEC SP-825 2 - 12 Usable solvents are listed below. (1) The standard eluents are buffers and aqueous solutions of salt such as sodium chloride, potassium chloride, sodium sulfate, and potassium sulfate.
- Page 4 Operation Manual Shodex IEC SP-825 Version 211129E • Always use freshly prepared solvents. Solvents stored for a long time may have changed their compositions and may influence elution patterns and/or damage the column. Note • Use of on-line degasser is recommended.
- Page 5 Operation Manual Shodex IEC SP-825 Version 211129E Attention! • Make sure not to let air bubbles enter the column while installing the column. The air bubbles may damage the column. • When restarting the system after column installation or after holding the eluent flow, start the system at less than 0.5 mL/min.
- Page 6 Operation Manual Shodex IEC SP-825 Version 211129E Column Storage Remove the column from the system after replacing the in-column solvent with the shipping solvent. Securely tighten the end caps and store the column at a location with stable temperature (a cool and dark space is recommended).
- Page 7 Version 211129E 11. Warranty (Version 4) (1) Showa Denko K.K. warrants that the Shodex™ product, at the time of delivery to the user, will conform to the specification of the attached CERTIFICATE OF ANALYSIS, if the Shodex product is used in accordance with the attached operating manual.
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