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（Please read this manual carefully to achieve the best and consistent column performance for a long time）
Important Handling Instructions
Caution!
· Please consult the Safety Data Sheet (SDS) of reagents and solvents used with the column
and understand their proper handling methods to prevent potential health hazards or death
from occurring.
· Please wear appropriate personal protective equipment such as lab goggles and gloves
when handling organic solvents and acid and alkaline reagents. Avoid any direct physical
contact to prevent chemical injuries.
Before Using the Column
(1) Please visually inspect the package and outside of the column for any damage.
(2) Please check if product name and serial number written on the column package, column name tag, and
enclosed Certificate of Analysis (CoA) are matching and correct.
1. Introduction
Thank you for purchasing the Shodex product. Shodex IC NI-424 is a non-suppressor type ion
chromatography column. It is suitable for separation and analysis of anions in the environmental field.
2. Column Components
3. Column Specifications
Product
Product Name
Code
F6995243
IC NI-424
F6709616
IC NI-G
: Spherical porous particles of polyhydroxymethacrylate modified with quaternary ammonium
Base Material
group
Column Housing : SUS-316
Screw Type
: Internally-threaded type No.10-32 UNF
Shipping Solvent : Aqueous Solution containing 8 mM 4-Hydroxybenzoic Acid + 2.8 mM Bis-Tris
+ 2 mM Phenylboronic Acid + 0.005 mM CyDTA
Operation Manual
Shodex
IC NI-424
Column Size (mm)
I.D.
4.6
4.6
SHOWA DENKO K.K. (https://www.shodex.com/)
Particle
Size (μm)
Length
100
5
10
5
*2
*1
Bis(2-hydroxyethyl)imino-tris-(hydroxymethyl) methane
*2
trans-1,2-Diaminocyclohexane-N,N,N',N'-tetraacetic acid
ver.211109E
Theoretical Plate Number
(Per Column)
≥ 5,000
(Guard Column)
*1
1
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Summary of Contents for Showa Denko Shodex IC NI-424
- Page 1 Column Housing : SUS-316 Screw Type : Internally-threaded type No.10-32 UNF Shipping Solvent : Aqueous Solution containing 8 mM 4-Hydroxybenzoic Acid + 2.8 mM Bis-Tris + 2 mM Phenylboronic Acid + 0.005 mM CyDTA Bis(2-hydroxyethyl)imino-tris-(hydroxymethyl) methane trans-1,2-Diaminocyclohexane-N,N,N',N'-tetraacetic acid SHOWA DENKO K.K. (https://www.shodex.com/)
- Page 2 The content may have been changed, absorbed moisture, or has been contaminated. · Always use freshly prepared solvents. Solvents stored for a long time may have changed their compositions and may influence elution patterns and/or damage the column. SHOWA DENKO K.K. (https://www.shodex.com/)
- Page 3 If the pump was stopped while the eluent inside the column was still hot, as the eluent temperature decreases, its volume also decreases. This may result in creating an empty space in the column and deteriorates the column. SHOWA DENKO K.K. (https://www.shodex.com/)
- Page 4 (a cool and dark space is recommended). Refer to section 7.3 Solvent Exchange for how to replace the eluent. Attention! · Never allow inside of the column to dry. It can damage the column. SHOWA DENKO K.K. (https://www.shodex.com/)
- Page 5 (2) Do not make a strong impact on the column. Do not drop or hit the column on a hard surface. Refer to the Shodex™ website (https://www.shodex.com/) for product details and their applications. For additional assistance, contact the distributor from whom you purchased the column or contact your regional Shodex support office (https://www.shodex.com/en/support_office/list/). SHOWA DENKO K.K. (https://www.shodex.com/)
- Page 6 11. Warranty (Ver. 4COLUMN) (1) Showa Denko K. K. warrants that the Shodex product, at the time of delivery to the user, will conform to the specification of the attached CERTIFICATE OF ANALYSIS, if the Shodex product is used in accordance with the attached operating manual.
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