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Operation Manual Shodex Asahipak GS-HQ series
Shodex™ Asahipak GS-HQ series
Europe, Middle East, Africa, Russia
www.shodex.de
Operation Manual
Sales Office:
Showa Denko Europe GmbH
Shodex Business
Konrad-Zuse-Platz 3
81829 Munich
Germany
Version 210629E
Technical Support:
info@shodex.de
+49 (0)89 / 93 99 62 37
Quotes and Orders:
order@shodex.de
+49 (0)89 / 93 99 62 34
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Summary of Contents for Showa Denko Shodex Asahipak GS-HQ Series
- Page 1 Operation Manual Shodex Asahipak GS-HQ series Version 210629E Operation Manual Shodex™ Asahipak GS-HQ series Sales Office: Technical Support: Showa Denko Europe GmbH info@shodex.de Shodex Business +49 (0)89 / 93 99 62 37 Konrad-Zuse-Platz 3 81829 Munich Quotes and Orders: Europe, Middle East, Africa, Russia Germany order@shodex.de...
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Page 2: Important Handling Instructions
Certificate of Analysis (CoA) are matching and correct. Introduction Thank you for purchasing the Shodex™ product. Shodex Asahipak GS-HQ series is a polymer-based size exclusion chromatography column. With the choice of eluent, the column provides multimode features of reversed phase, HILIC, and ion exchange modes to SEC. It is suitable for the separations of peptides or nucleic acids with similar molecular weights. - Page 3 Operation Manual Shodex Asahipak GS-HQ series Version 210629E Product Code Product Name Target Molecular Weight Range Exclusion Limit F7600005 Asahipak GS-220 HQ 300 – 3,000 7,000 F7600006 Asahipak GS-320 HQ 300 - 20,000 40,000 F7600007 Asahipak GS-520 HQ * 5,000 - 200,000...
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Page 4: Eluent Preparation
Operation Manual Shodex Asahipak GS-HQ series Version 210629E column temperature so that the column pressure remains below the usable maximum pressure. • Shear degradation is more likely to occur in larger molecular weight compounds. When shear degradation occurs, the measured molecular weight may be lower than the actual value or it may influence the repeatability of the analysis. -
Page 5: Column Installation
Operation Manual Shodex Asahipak GS-HQ series Version 210629E Column Usage Procedure 7.1. HPLC System Preparation Wash entire LC system prior to the column installation, including all flow-lines and sample loop by switching the valve, and then replace the washing solution with the eluent to be used. If desired new eluent has low miscibility/solubility to the eluent of previous analysis, first use the eluent that is miscible/soluble to both eluents, and then replace it with the desired eluent. -
Page 6: Column Cleaning
Operation Manual Shodex Asahipak GS-HQ series Version 210629E Note • It is recommended to set the pump limiter to avoid exceeding the maximum pressure. 7.3. Solvent Exchange (1) When replacing the solvent, start the system at less than 0.2 ml/min. Recommended solvent volume to introduce at each step is 3 to 5 times of the column volume. -
Page 7: Column Storage
Operation Manual Shodex Asahipak GS-HQ series Version 210629E Column Storage Remove the column from the system after replacing the in-column solvent with the shipping solvent. Securely tighten the end caps and store the column at a location with stable temperature (a cool and dark space is recommended). - Page 8 3-3 If the Shodex product is disposed of 3-4 If the Shodex product is resold by the user without giving prior written notice to Showa Denko K.K. 3-5 If the performance of the Shodex product is not conform to the specification of the attached CERTIFICATE OF ANALYSIS due to any of the reasons below: a.
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