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Operation Manual Shodex GPC LF-404
Europe, Middle East, Africa, Russia
www.shodex.de
Operation Manual
Shodex™ GPC LF-404
Sales Office:
Showa Denko Europe GmbH
Shodex Business
Konrad-Zuse-Platz 3
81829 Munich
Germany
Version 211001E
Technical Support:
info@shodex.de
+49 (0)89 / 93 99 62 37
Quotes and Orders:
order@shodex.de
+49 (0)89 / 93 99 62 34
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Summary of Contents for Showa Denko Shodex GPC LF-404
- Page 1 Operation Manual Shodex GPC LF-404 Version 211001E Operation Manual Shodex™ GPC LF-404 Sales Office: Technical Support: Showa Denko Europe GmbH info@shodex.de Shodex Business +49 (0)89 / 93 99 62 37 Konrad-Zuse-Platz 3 81829 Munich Quotes and Orders: Europe, Middle East, Africa, Russia Germany order@shodex.de...
- Page 2 Certificate of Analysis (CoA) are matching and correct. Introduction Thank you for purchasing the Shodex™ product. Shodex GPC LF-404 is a size exclusion chromatography semi-micro column for high performance analysis used with organic solvents. It is packed with unique multi-pore gels with a wide pore-size distribution.
- Page 3 Operation Manual Shodex GPC LF-404 Version 211001E Usable Conditions 4.1 System Settings Flow Rate (mL/min) Temperature (°C) Maximum Pressure Product Name (MPa) (Per Column) Recommended Maximum Recommended Range GPC LF-404 25 - 40 20 - 60 GPC LF-G 4.2 List of Applicable Solvents...
- Page 4 Operation Manual Shodex GPC LF-404 Version 211001E Eluent Preparation (1) Degas the eluent fully to prevent the formation of air bubbles. (2) Presence of small debris or insoluble substances may result in deterioration of the column and/or they appear as noise on the chromatograms. Filter the eluent with a 0.45 μm disposable filter to prevent the problems.
- Page 5 Operation Manual Shodex GPC LF-404 Version 211001E Column Usage Procedure GPC LF-404 is a semi-micro column. To achieve its best performance, please use it with a semi-micro type system. 7.1. HPLC System Preparation Wash entire LC system prior to the column installation, including all flow-lines and sample loop by switching the valve, and then replace the washing solution with the eluent to be used.
- Page 6 Operation Manual Shodex GPC LF-404 Version 211001E 7.3. Solvent Exchange (1) When replacing the solvent, set the column temperature at 40 - 60°C and start the system at less than 0.1 mL/min. Recommended solvent volume to introduce at each step is 3 to 5 times of the column volume.
- Page 7 Operation Manual Shodex GPC LF-404 Version 211001E Column Inspection Inspection method is described in the Certificate of Analysis (CoA). Theoretical Plate Number (N) and Asymmetry Factor (Fas) were calculated using the below equations. (1) Theoretical Plate Number (N) (2) Asymmetry Factor (Fas) 10.
- Page 8 3-3 If the Shodex product is disposed of 3-4 If the Shodex product is resold by the user without giving prior written notice to Showa Denko K.K. 3-5 If the performance of the Shodex product is not conform to the specification of the attached CERTIFICATE OF ANALYSIS due to any of the reasons below: a.
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