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Operation Manual Shodex RSpak JJ-50 2D
Shodex™ RSpak™ JJ-50 2D
Europe, Middle East, Africa, Russia
www.shodex.de
Operation Manual
Sales Office:
Showa Denko Europe GmbH
Shodex Business
Konrad-Zuse-Platz 3
81829 Munich
Germany
Version 200407E
Technical Support:
info@shodex.de
+49 (0)89 / 93 99 62 37
Quotes and Orders:
order@shodex.de
+49 (0)89 / 93 99 62 34
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Summary of Contents for Showa Denko Shodex RSpak JJ-50 2D
- Page 1 Operation Manual Shodex RSpak JJ-50 2D Version 200407E Operation Manual Shodex™ RSpak™ JJ-50 2D Sales Office: Technical Support: Showa Denko Europe GmbH info@shodex.de Shodex Business +49 (0)89 / 93 99 62 37 Konrad-Zuse-Platz 3 81829 Munich Quotes and Orders: Europe, Middle East, Africa, Russia Germany order@shodex.de...
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Page 2: Important Handling Instructions
Operation Manual Shodex RSpak JJ-50 2D Version 200407E Operation Manual Shodex RSpak JJ-50 2D (Please read this manual carefully to achieve the best and consistent column performance for a long time) Important Handling Instructions Caution! • Please consult the Safety Data Sheet (SDS) of reagents and solvents used with the column and understand their proper handling methods to prevent potential health hazards or death from occurring. -
Page 3: Sample Preparation
Operation Manual Shodex RSpak JJ-50 2D Version 200407E Column Specifications Theoretical Plate Product Functional Particle Pore Columns Size Product Name Number Code group Size size ID x Length (per column) Quaternary F7008220 RSpak JJ-50 2D ≥ 3,500 5 μm 100 Å 2.0 x 150 mm... -
Page 4: Column Installation
Operation Manual Shodex RSpak JJ-50 2D Version 200407E Column Usage Procedure RSpak JJ-50 2D is a semi-micro column. To achieve its best performance, please use it with a semi- micro type system. 6.1. HPLC System Preparation Wash entire LC system prior to the column installation, including all flow-lines and sample loop by switching the valve, and then replace the washing solution with the eluent to be used. -
Page 5: Column Cleaning
Operation Manual Shodex RSpak JJ-50 2D Version 200407E 6.3. Eluents (1) Degas the eluent fully to prevent the formation of air bubbles. (2) Presence of small debris or insoluble substances may result in deterioration of the column and/or they appear as noise on the chromatograms. Filter the eluent with a 0.45 μm disposable filter to prevent the problems. -
Page 6: Column Storage
Operation Manual Shodex RSpak JJ-50 2D Version 200407E Method 1: Adsorption of hydrophilic compounds Washing Solution Cleaning Time 6 min 0.1 M HNO aq. solution 75 min 12 min Eluent 40 min Method 2: Adsorption of metal ions or in case Method 1 did not work... -
Page 7: Additional Warnings
Operation Manual Shodex RSpak JJ-50 2D Version 200407E Column Inspection Inspection method is described in the Certificate of Analysis (CoA). Theoretical Plate Number (N) and Asymmetry Factor (Fas) were calculated using the below equations. (1) Theoretical Plate Number (N) (2) Asymmetry Factor (Fas) Additional Warnings (1) Do not remove end fittings. - Page 8 3-3 If the Shodex product is disposed of 3-4 If the Shodex product is resold by the user without giving prior written notice to Showa Denko K.K. 3-5 If the performance of the Shodex product is not conform to the specification of the attached...
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