Shodex RSpak JJ-50 2D Operation Manual

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(Please read this manual carefully to achieve the best and consistent column performance for a long time)
Important Handling Instructions
Caution!
· Please consult the Safety Data Sheet (SDS) of reagents and solvents used with the column
and understand their proper handling methods to prevent potential health hazards or death
from occurring.
· Please wear appropriate personal protective equipment such as lab goggles and gloves
when handling organic solvents and acid and alkaline reagents. Avoid any direct physical
contact to prevent chemical injuries.
Before Using the Column
(1) Please visually inspect the package and outside of the column for any damage.
(2) Please check if product name and serial number written on the column package, column name tag, and
enclosed Certificate of Analysis (CoA) are matching and correct.

1. Introduction

Thank you for purchasing the Shodex product. Shodex RSpak JJ-50 2D is a multimode (reversed phase
and anion exchange modes) column filled with spherical porous particles of polyvinyl alcohol modified with
quaternary ammonium group. It is an ideal column for analyzing complex samples that contain mixture of neutral
and ionic substances.

2. Column Components

3. Column Specifications

Product
Product Name
Code
F7008220
RSpak JJ-50 2D
Base Material
: Spherical porous particles of polyvinyl alcohol modified with quaternary ammonium group
Column Housing : SUS-316
Screw Type
: Internally-threaded type No.10-32 UNF
Shipping Solvent : Water/Acetonitrile = 40/60
Operation Manual
Shodex
RSpak
Column Size (mm)
I.D.
Length
2.0
150
SHOWA DENKO K.K. (https://www.shodex.com/)
JJ-50 2D
Particle Size
Theoretical Plate Number
(μm)
5
ver.211124E
(Per Column)
≥ 3,500
1

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Summary of Contents for Shodex RSpak JJ-50 2D

  • Page 1 Certificate of Analysis (CoA) are matching and correct. 1. Introduction Thank you for purchasing the Shodex product. Shodex RSpak JJ-50 2D is a multimode (reversed phase and anion exchange modes) column filled with spherical porous particles of polyvinyl alcohol modified with quaternary ammonium group.
  • Page 2 (3) Recommended sample injection volume is less than 5 μL per column. 7. Column Usage Procedure RSpak JJ-50 2D is a semi-micro column. To achieve its best performance, please use it with a semi-micro type system. 7.1 HPLC System Preparation Wash entire LC system prior to the column installation, including all flow-lines and sample loop by switching the valve, and then replace the washing solution with the eluent to be used.
  • Page 3 (Refer to section 7.4 Column Cleaning, Method 1). (6) When using a gradient method, changes in the eluent compositions may increase the column backpressure. Adjust the flow rate and column temperature so that the column backpressure remains below the usable maximum pressure. SHOWA DENKO K.K. (https://www.shodex.com/)
  • Page 4 (a cool and dark space is recommended). Refer to section 7.3 Solvent Exchange for how to replace the eluent. Attention! · Never allow inside of the column to dry. It can damage the column. SHOWA DENKO K.K. (https://www.shodex.com/)
  • Page 5 (2) Do not make a strong impact on the column. Do not drop or hit the column on a hard surface. Refer to the Shodex™ website (https://www.shodex.com/) for product details and their applications. For additional assistance, contact the distributor from whom you purchased the column or contact your regional Shodex...
  • Page 6 11. Warranty (Ver. 4COLUMN) (1) Showa Denko K. K. warrants that the Shodex product, at the time of delivery to the user, will conform to the specification of the attached CERTIFICATE OF ANALYSIS, if the Shodex product is used in accordance with the attached operating manual.

This manual is also suitable for:

F7008220

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