Running A Thermal Paper Test And Adjusting The Tilt Micrometer; Pulling A Pipette - Sutter Instrument P2000/G Quick Start Manual

6. Advance both puller bars completely forward using the finger holds. A puller bar is
completely advanced when the slot cut in the bar touches the edge of the stop screw
in that slot.
7. Holding the puller bars together with one hand, slightly loosen the clamp holding the
glass, so the glass can be advanced without falling out of the top groove of the bar.
8. Advance the glass until an even length of glass is on either side of the
shroud/retromirror.
9. Still holding the bars together, lock the glass in this position by tightening the clamps
on both bars. Tighten the clamps snugly. Do not overtighten.

Running a Thermal Paper Test and Adjusting the Tilt Micrometer

1. Remove the shroud by loosening the two screws which hold it in place and lifting it straight up.
2. Load a piece of glass, preferably one with a 1 mm OD.
3. Place a piece of thermal paper between the glass and the metal retromirror with the heat-sensitive side of the paper facing the
glass. The heat-sensitive side of the paper is slightly glossy while the insensitive size has clearly visible red inclusions.
4. Access program 51.
5. Close the lid and press the green <PULL> button. The laser will not fire if the lid is open.
6. Remove the thermal paper without unloading the glass. You should see a burn mark with two lobes. These should be of equal
size. If the upper lobe is larger, turn the tilt micrometer counterclockwise less than ¼ turn and repeat the test. If the lower lobe is
larger, turn the micrometer clockwise. If there is only one elliptical burn mark with a lighter grey or white center, the laser is
completely missing the glass. Refer to the label on the back of the instrument and reset the tilt micrometer to this factory setting.
7. Repeat steps 3-6 until both lobes are of equal size.
8. Clean the retromirror as outlined in the maintenance section.

Pulling a Pipette

1. Access a program of your choice. Consult the manual before designing your own programs to understand the parameters.
Programming advice can be obtained by contacting Sutter technical support (info@sutter.com)
2. Load a piece of glass, close the cover, and press <PULL>. The puller will run through the programmed lines in order. If the glass
fails to separate, the program will loop back to the first line and start again until a pipette is pulled. Refer to the manual for
information on looping and multiple line programs. If the laser is on continuously for more than 50 seconds the program will time
out. This usually indicates either incorrect loading of glass or a bad program.
3. After the glass has separated, but before opening the lid, the puller will display information about the pull (number of loops, last
line used, and heat-on time). For a stable program, the first 2 values should not vary between pulls, and the heat-on time should
not vary by more than 1 second.
Sensitive Side
Good Laser too high. Turn
adjustment micrometer
counterclockwise
Step 5
Step 7
Step 8
Insensitive Side
Laser Too Low. Complete miss.
Turn micrometer Reset micrometer
clockwise to factory default
Stop Screw