Separation Of Dna Strands And Release Of Samples Into The Pyromark Q24 Plate - Qiagen PyroMark Q24 MDx User Manual

5.3.4 Separation of DNA strands and release of
samples into the PyroMark Q24 Plate
WARNING Hazardous chemicals
The Denaturation Solution used with the vacuum
workstation contains sodium hydroxide, which is irritating
to eyes and skin.
Always wear safety glasses, gloves, and a lab coat.
The responsible body (e.g., laboratory manager) must take
the necessary precautions to ensure that the surrounding
workplace is safe and that the operators are not exposed
to hazardous levels of toxic substances (chemical or
biological) as defined in the applicable Material Safety
Data Sheets (MSDSs) or OSHA,* ACGIH,
documents. For more information, visit
www.qiagen.com/support/msds.aspx.
Venting for fumes and disposal of wastes must be in
accordance with all national, state, and local health and
safety regulations and laws.
* OSHA: Occupational Safety and Health Administration (United States of America).
† ACGIH: American Conference of Government Industrial Hygienists (United States of America).
‡
COSHH: Control of Substances Hazardous to Health (United Kingdom).
Things to do before starting
Prewarm one of the supplied PyroMark Q24 Plate Holders to
be used in Section 5.3.5 by placing it (without a plate) on a
heating block at 80°C.
Procedure
1. Ensure that the PyroMark Q24 MDx Vacuum Workstation
PyroMark Q24 MDx User Manual 01/2016
Note: Sepharose beads sediment quickly and capturing
of beads must take place immediately once agitation is
complete.
Note: During immobilization, prepare the vacuum
workstation for the sample preparation (steps 1–8 in
Section 5.3.4).
has been assembled correctly and securely.
Operating Procedures
† or COSHH ‡
5-11