Checking The Volume - BRAND Transferpette S Operating Manual

5 Checking the volume

5 Checking the volume
We recommend testing the device every 3 to 12 months, depending on
the level of use. However, the testing cycle can be adapted to meet indi-
vidual requirements. Gravimetric volume testing of the pipette is carried
out according to the following steps and complies with DIN EN ISO 8655,
Part 6.
1. Setting the nominal volume
a.
Set the maximum specified instrument volume (for procedure, see
Pipetting, p. 43 ).
2. Conditioning the pipette
a.
Condition the pipette before testing by aspirating and dispensing the
test liquid (distilled water) with a pipette tip five times.
3. Performing the test
In accordance with DIN EN ISO 8655-2, a tip change is recommended af-
ter each individual measurement. An exception to this rule can be made,
according to DAkkS guideline DKD-R8-1.
a.
Aspirate the test liquid and pipette into the weighing vessel.
b.
Weigh the pipetted amount with an analysis scale. (refer to the oper-
ating manual of the balance manufacturer.)
c.
Calculate the pipetted volume. In doing so, take into account the tem-
perature of the test liquid.
d.
At least 10 pipetting series and weighings in 3 volume ranges (100 %,
50 %, 10 %) are recommended.
Operating manual
46
NOTICE
Transferpette® S 997207