Incubator; Sterility; Humidity - NuAire 12 Series Operation And Maintenance Manual

Air-jacketed dh invitrocell co2 incubator
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1.0
General Description
The NuAire Direct Heat (DH) Automatic CO
vitro environment for optimum tissue cell culture growth. The chamber also provides an environment for the storage and
preservation of embryos, gametes and animal tissue cell cultures intended for research at or near body temperature. There
are five parameters that contribute to optimum growth conditions.
These are:

Humidity

Precise temperature control
Precise CO

Sterility

Reliability
Like all NuAire equipment, this Incubator has been designed to provide the highest quality standards of performance with
matching computer technology, precise temperature control, and CO
technology with years of design, quality, and manufacturing experience.
1.1 Incubator Chamber
The design and size of the DH inner chamber provides a large capacity (7 cu. ft.), and ease of use. The chamber walls are
directly heated by physically attached foil heating elements on the sides, bottom, top, and back of the chamber, providing a
temperature uniformity of +0.3°C. A space-age high-density insulation that has a high "R" rating covers the complete outer
surfaces of the Incubator inner chamber.
1.2 Incubator Closed-loop chamber sample circulation system & HEPA Filter
A continuous operating air pump draws environmental sample from the chamber and circulates it through a HEPA filter
capsule then through sensors that measure CO2 volume (as a percentage) in the chamber for monitoring and control.
1.3 Incubator Control Electronics
The NuAire Incubator Control Electronics is a state-of-the-art microcomputer-based control system specifically designed to
service the precise control requirements of the chambers environment, providing optimum programmable conditions for
culture growth. The microcomputer is "user-friendly" and provides a 5x7 LCD screen which provides operating control
parameters, status indicators, additional key operational parameters, and an imbedded touch panel to permit efficient
operator entry of data.
The microcomputer is supported with Read-Only Memory (ROM) containing executable software, Random Access Memory
(RAM) for temporary storage, and Electronically Erasable Programmable Read-Only Memory (EEPROM) for control set
points and parameters. The EEPROM provides for indefinite storage of these values during periods of power off or power
interruption (power fault-tolerant).
1.4 Incubator CO
Control
2
The NuAire direct heat Incubator incorporates a microprocessor-based, non-dispersive infrared CO
The amount of energy received at the detector is an approximate logarithmic function of the CO
between source and detector. The wavelengths used are absorbed only by CO
other components, such as water vapor. Detector linearization is performed with 32-bit digital accuracy. Advanced design
provides a very stable output minimizing drift and requiring less frequent calibration. The output is digital, alleviating errors
brought about by analog signals. Calibration of this control is accomplished by a zero & span calibration done on the sensor
and a display off- set calibration. These calibrations are accessible through the NU-TOUCH LCD located on the front of the
unit.
OM0245
Rev 6 March/2022
Models: NU-5800 and NU-5800E
Operation and Maintenance Manual
Direct Heat Incubator has been designed to provide a reliable controlled in-
2
control
2
DH Direct Heat
Automatic CO

Incubator

2
gas control system combining state-of-the-art
2
Page 3 of 53
induction sensor.
2
concentration in the gas
2
making the measurement insensitive to
2

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Nu-5800Nu-5800e

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