Omni PCR-258 User Manual
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Omni PCR-258 User Manual

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OMNI Tip™
HOMOGENIZING KITS
USER MANUAL
REV. B
PCR-258

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Summary of Contents for Omni PCR-258

  • Page 1 OMNI Tip™ HOMOGENIZING KITS USER MANUAL REV. B PCR-258...

  • Page 2: Warranty Information

    WARRANTY INFORMATION This manual is a guide for the use of the Omni International’s Omni Tip™ Homogenizing Kits and accessories. Data herein has been verified. It is believed adequate for the intended use of the instrument. If the instrument or procedures are...

  • Page 3: Important Safeguards And Warnings

    Do not attempt to modify any part of the Omni TH. If you experience problems with or have questions about your Omni TH, contact your authorized dealer or call Omni International at 1-800-776-4431 or 1-770-421-0058.
  • Page 4 CAUTION: Large, solid particles, such as frozen tissue, may cause damage to the standard Omni Tip™ generator probes. Use the Hard Tissue Omni Tips™ for those applications. WARNING: Keep all housings in place and in working order.

  • Page 5: Table Of Contents

    Section 1 — Omni Tip™ Kits 1.1 Omni Tip Kits ..................6 1.2 Accessories ..................... 7 Section 2 — Omni TH, Tissue Homogenizer 2.1 Specifications ..................8 2.2 Parts and Accessories ................. 8 Section 3 — Stand-Mounting the Omni TH 3.1 Stand-Mounting the Omni TH ............

  • Page 6: Section 1 - Omni Tip™ Kits

    Omni’s convenient Tissue Homogenizing Kits come with everything needed to homogenize tissue samples quickly and efficiently for successful RNA extraction. Each kit includes an Omni TH Homogenizer with an adapter for mounting Omni Tips™ generator probes (see Omni Tip™ Plastic Probes Section for more information). A choice of stainless steel rotor-stator generator probes is also available (see Motor Maintenance Section for more information).

  • Page 7: Accessories

    Stand Assembly Kit Additional Omni Tip™ generator probes are available. Omni Tips™ are 110 mm long and 7 mm in diameter. The adapter included in Omni’s Tissue Homogenizing Kits is required for mounting Omni Tips™ to the Omni TH Homogenizer, and is not disposable. See Section 5 for more information about Omni Tips™.

  • Page 8: Section 2 - Omni Th, Tissue Homogenizer

    T1001 Instruction Manual PCR-258 Adapter for Omni Tips 12-120 A stand assembly (base plate, 24” post, cross rod, and post clamp), P/N S1000, is available for use with the Omni TH, Tissue Homogenizer, but is not supplied with the instrument.

  • Page 9: Section 3 - Stand-Mounting The Omni Th

    Omni TH motor by means of a quarter-turn bayonet mount. Simply push the generator probe into the motor housing as far as possible, turn clockwise, and release. Remove the blue protective cap from the tip of the generator probe and the Omni TH is ready to operate.

  • Page 10: Omni Tip™ Plastic Probes

    Probes with the Omni TH. Omni TH Tissue Homogenizers with a black nose ring will require Omni Tip adapter (A1000SB) to use Omni Tips. First, insert the smaller end of the Omni Tip Adapter into the Omni TH as far as possible, turn clockwise, and release. Next, insert an Omni Tip Plastic Probe into the Omni Tip Adapter as far as possible, turn clock- wise, and release.

  • Page 11: Theory Of Operation

    SECTION 3 — OMNI TH OPERATION 3.5 THEORY OF OPERATION The rotor shaft is coupled directly to the drive motor, via the drive pin. When attached to the homogenizer motor, the rotor shaft can spin up to 35,000rpm. This assembly makes up the rotor portion of the rotor-stator generator probe.

  • Page 12: Section 4 - Operational Guidelines

    Non-sterile, plastic Omni Tips™ generator probes are ideal for situations where molecular contamination between samples cannot be tolerated. Omni Tips™ were designed to be disposed of after each use, eliminat- ing cross contamination risks along with the time and effort normally required for cleaning.
  • Page 13 Solid particles, in any dimension, should be no more than half the diameter of the rotor-stator generator probe for optimal process- ing. This is extremely important when using the Omni Tip™ dis- posable generator probes. Stainless steel generator probes with extended blades and/or wide windows are available for processing large, solid samples.

  • Page 14: Section 5 -Omni Tip™ Plastic Probes

    Length: 110 mm The Omni Tips™ are disposable rotor-stator generator probes constructed of durable plastic. Although Omni Tips™ are designed specifically to be dis-posed of after each use to eliminate sample cross-contamination, for many applica- tions, the probe can be cleaned, sterilized, or disinfected and reused by the procedures described below.

  • Page 15: Installing Omni Tips

    PCR studies, are not recommended for extended use with Omni Tips™. Exposure to these chemicals shortens the life of Omni Tips™ and usually results in disposal after each use. Therefore, reuse is not guaranteed in such circumstances.

  • Page 16: Autoclaving Omni Tips

    The tube and shaft of Omni Tips™ can be autoclaved at up to a maximum of 250°F for 45 minutes. This includes a 15-minute ramp up, 15-minute hold, and a 15-minute ramp down of the temperature cycle.

  • Page 17: Section 6 - Service

    1–800–776-4431 or online at www.omni-inc.com. SECTION 7 — TROUBLESHOOTING Do not attempt to service the Omni TH in a manner other than those discussed in this manual. For any issue that is unsuccessfully corrected using this guide, please contact your authorized dealer or call Omni International technical assistance at 1-800-776-4431 or 1-770-421-0058.
  • Page 18 NOTES:...
  • Page 19 NOTES:...
  • Page 20 OMNI Tip™ HOMOGENIZING KITS Omni International, Inc. 935C Cobb Place Blvd. Kennesaw, GA 30144 800.776.4431  770.421.0058  www.omni-inc.com...

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