Section 4 - Operational Guidelines - Omni PCR-258 User Manual

SECTION 4 — OPERATIONAL GUIDELINES
4.1 OPERATIONAL GUIDELINES
Rotor-stator homogenization provides highly efficient cellular lysis and
membrane disruption while reducing the high-molecular weight of
cellular components. Omni Tip Tissue Homogenizing Kits allow rapid
and complete disruption of cell lysates to expose intracellular contents
and ensure increased yields. A choice of rotor-stator generator probes,
in plastic or stainless steel, process a wide range of sample preparation
applications.
Non-sterile, plastic Omni Tips™ generator probes are ideal for situations
where molecular contamination between samples cannot be tolerated.
Omni Tips™ were designed to be disposed of after each use, eliminat-
ing cross contamination risks along with the time and effort normally
required for cleaning. While Omni Tips™ can be autoclaved up to seven
times for repeated use, they are not recommended for extended use
with phenol or guanidine-based reagents (see Section 5.3 for chemi-
cal resistance compatibility). Disposal may be required when used in
conjunction with these reagents.
Stainless steel generator probes are best for difficult-to-process tissues
and for situations where sample-to-sample contamination is not a
concern. Stainless steel generator probes are equipped with a Teflon
bearing. Omni's stainless steel generator probes can be quickly disas-
sembled for thorough cleaning and bearing maintenance.
Omni's stainless steel generator probes are available in many con-
figurations tailored for specific sample types. Flat bottom generator
probes are recommended for liquid or soft tissues, while saw tooth and
extended blades are best for processing fibrous tissues. For situations
where preprocessing or cutting the tissue prior to homogenization is
inconvenient, wide windows allow larger pieces of sample to effective-
ly pass through the processing head where shearing occurs.
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