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NEW BRUNSWICK SCIENTIFIC MP-1000 Pourmatic Manual To Operations

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MP-1000 Pourmatic
Automated Plate Pourer
MANUAL No. M1224-0050
Revision Q
September 22, 2005
NEW BRUNSWICK SCIENTIFIC CO., INC.
BOX 4005 • 44 TALMADGE ROAD • EDISON, NJ 08818-4005
Telephone: 1-732-287-1200 • 1-800-631-5417
Fax: 732-287-4222 • Telex: 4753012 NBSCO
Internet: http://www.nbsc.com • E-mail: bioinfo@nbsc.com

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  • Page 1 Automated Plate Pourer MANUAL No. M1224-0050 Revision Q September 22, 2005 NEW BRUNSWICK SCIENTIFIC CO., INC. BOX 4005 • 44 TALMADGE ROAD • EDISON, NJ 08818-4005 Telephone: 1-732-287-1200 • 1-800-631-5417 Fax: 732-287-4222 • Telex: 4753012 NBSCO Internet: http://www.nbsc.com • E-mail: bioinfo@nbsc.com...
  • Page 2 MP-1000 PourMatic M1224-0050 User’s Guide...
  • Page 3 Fax: 49 (0)7022 32486 E-mail: sales@nbsnv-sa.be E-mail: sales@nbsgmbh.de CHINA THE NETHERLANDS New Brunswick Scientific Co., Inc. New Brunswick Scientific BV Room 1501, Xiangjiang Building, No. 18 Kerkenbos 1101, 6546 BC Nijmegen Lane 1265, Zhongshan Road (W) P.O Box 6826, 6503 GH Nijmegen Shanghai 200051, P.R.
  • Page 4 CAUTION! For optimum operation of this unit, please restrict your use of petri dishes to the following only: 60 x 15mm Falcon 1007 Fisher 8-757-13A 100 x 15mm Falcon 1029 Fisher 8-757-13 Lab-Tek 4002 MP-1000 PourMatic M1224-0050 User’s Guide...
  • Page 5 Reproduction, adaptation, or translation without prior written permission from New Brunswick Scientific is prohibited. Disclaimer Notice New Brunswick Scientific Co., Inc. reserves the right to change information in this document without notice. Updates to information in this document reflect our commitment to continuing product development and improvement.
  • Page 6 W A R R A N T Y Every Instrument manufactured by the New Brunswick Scientific Co., Inc. is warranted to be free from defects in material and work- manship. This apparatus with the exception of glassware, lamps and electrodes (where supplied), is warranted for 1 year against faulty com-...

  • Page 7: Table Of Contents

    ATCH ....................29 ANUAL & S .................29 OZZLE SSEMBLY TERILIZATION ................30 OADING UBING INTO 7.10 ..................31 NSTALLING THE OVER OPERATION ........................33 ...........................33 PEED ........................33 .......................34 RIMING THE ........................35 ALIBRATION ................36 NTERING A ISPENSING OLUME New Brunswick Scientific Co., Inc. User’s Guide...
  • Page 8 .....................52 EPLACEMENT TROUBLESHOOTING ....................54 13.1 ....................54 RROR ESSAGE ABLE REPLACEMENT PARTS & ACCESSORIES............56 14.1 ....................56 EPLACEMENT ARTS 14.2 ........................56 CCESSORIES 14.3 ......................56 ONVERSION DRAWINGS ........................60 15.1 ....................60 ONTROL CHEMATICS 15.2 ....................62 NDEX OF RAWINGS INDEX..........................63 MP-1000 PourMatic M1224-0050 User’s Guide...

  • Page 9: Overview

    Automatic shut-off occurs as audible and visible signals alert the user of an upside-down, half or jammed dish. As a safety feature, the germicidal lamp is controlled by an interlock switch; it lights only when the dust cover is properly positioned. New Brunswick Scientific Co., Inc. User’s Guide...

  • Page 10: 1.1 Specifications

    Sterility See-through Dust Cover and Germicidal Lamp help eliminate dust and contamination Weight 80 lbs. Electrical Requirements 100/120V—50/60 Hz, 480VA 220/240V—50/60 Hz, 480VA Regulatory Standards CE Declaration of Conformity See the following page. MP-1000 PourMatic M1224-0050 User’s Guide...
  • Page 11 New Brunswick Scientific Co., Inc. User’s Guide...

  • Page 12: Inspection & Verification

    & Inspection of Boxes After you have received your order from New Brunswick Scientific, inspect the boxes carefully for any damage that may have occurred during shipping. Report any damage immediately to your local NBS Sales Order Department or local NBS distributor.

  • Page 13: Preparing The Location

    NOTE: The Environmental Operating Range of 5 to 40°C is the range of temperatures at which the PourMatic can operate. Successful operation is subject to the agar remaining liquid throughout the dispensing process. New Brunswick Scientific Co., Inc. User’s Guide...

  • Page 14: Unpacking & Inspection

    Save all packing materials and User’s Guide. If any part of your order was damaged during shipping, missing pieces or fails to operate properly, please fill out the Customer Service Form 6300 and return by fax. You can also call the New Brunswick Scientific Service Department.

  • Page 15: Inspection Of Equipment

    CAROUSEL SECURING KNOB installed on PourMatic Base IEC-320 DETACHABLE POWER CORD PourMatic CAROUSEL 2 DISPENSING NOZZLES 1 installed on PourMatic 1 loose TRANSPARENT DUST COVER installed on PourMatic GERMICIDAL LAMP installed on PourMatic New Brunswick Scientific Co., Inc. User’s Guide...
  • Page 16 12 FT. SILICONE TUBING CATCH TRAY 2 HOSE CLAMPS SPARE FUSE 25 ml. GRADUATED CYLINDER SPILL TUBE PourMatic USER’S GUIDE PourMatic User’s Guide MP-1000 PourMatic M1224-0050 User’s Guide...

  • Page 17: Features

    Agar runs through tubing from reservoir to petri dishes • PERISTALTIC PUMP Roller pump for dispensing agar • Diverts spilled agar to the SPILL TUBE CATCH TRAY • Catches spilled agar & is removable CATCH TRAY New Brunswick Scientific Co., Inc. User’s Guide...
  • Page 18 Used to select Select Key CALIBRATE DISPENSE , or SELECT VOLUME COOL TIME SPEED % parameters Increases the Setpoint of a Up Key displayed parameter Decreases the Setpoint of a Down Key Displayed parameter MP-1000 PourMatic M1224-0050 User’s Guide...
  • Page 19 Carousel Transfer Key CAROUSEL after a jam– illuminates CAROUSEL red when jams occur Rotates –Used Dish Transfer Key DISH TRANSFER TABLE to index the DISH TRANSFER TABLE after a jam– illuminates red when jams occur New Brunswick Scientific Co., Inc. User’s Guide...
  • Page 20 User adjust the speed of the Speed % DISH TRANSFER TABLE Figure 1: Function Indicators & Keys Function Function Display Indicators Function Keys Function Keys MP-1000 PourMatic M1224-0050 User’s Guide...
  • Page 21 Figure 2: PourMatic Carousel Stationary Carousel Securing Platform Knob Dispensing Nozzle Dish Transfer Table Peristaltic Pump Dust Cover Dish Sensors Spill Tube ON / OFF Rocker Switch Catch Tray Dispensing Nozzle Holder New Brunswick Scientific Co., Inc. User’s Guide...

  • Page 22: Setting Up

    , clockwise or counterclockwise until the bubble on the FEET STATIONARY is centered. PLATFORM 3. When the bubble is centered, secure the by tightening the FEET LOCKING NUTS against the base. Figure 3: Leveling Base Locking Nut Foot MP-1000 PourMatic M1224-0050 User’s Guide...

  • Page 23: Install Spill Tube

    PourMatic. To attach the CAROUSEL CAROUSEL 1. Unscrew the from the of the PourMatic. CAROUSEL SECURING KNOB BASE 2. Place the on the and verify that the is level. CAROUSEL BASE CAROUSEL New Brunswick Scientific Co., Inc. User’s Guide...

  • Page 24: Inserting The Catch Tray

    CATCH TRAY 1. Insert the in-between the located on the bottom CATCH TRAY CATCH TRAY GUIDES front of the PourMatic. 2. Slide the in until the back of the tray hits the stops. CATCH TRAY MP-1000 PourMatic M1224-0050 User’s Guide...

  • Page 25: Getting Started

    PourMatic and is a universal power-entry device SELECTOR that can adapt to worldwide power requirements. If you need to modify the voltage configuration, you must call New Brunswick Scientific. Making changes to the voltage requires changing components. Making Electrical Connection...

  • Page 26: Carousel Loading For Continuous And Batch Modes

    CONTINUOUS To prepare for operation in mode, follow these intructions: CONTINUOUS CONTINUOUS 1. Press the to select the CONTINUOUS / BATCH KEY CONTINUOUS BATCH mode. The indicator light illuminates. CONTINUOUS MP-1000 PourMatic M1224-0050 User’s Guide...

  • Page 27: Batch Mode

    If the PourMatic detects an empty column, it will stop. An alarm sounds when the cycle is complete. mode fills all eight stacks of petri dishes and BATCH then waits for you to remove and re–stack all the columns of the CAROUSEL New Brunswick Scientific Co., Inc. User’s Guide...
  • Page 28 When a station is fully loaded, there will be a gap of about 2 1/2″ from the highest dish to the top of the . DO NOT OVERLOAD. CAROUSEL MP-1000 PourMatic M1224-0050 User’s Guide...

  • Page 29: Auto / Manual Key

    HOSE CLAMP 4. Wrap the in aluminum foil. Place DISPENSING NOZZLE TUBING NOZZLE in an autoclave. Ensure that you use standard sterilizing techniques. TUBING 5. After sterilization is complete, remove from autoclave. NOZZLE New Brunswick Scientific Co., Inc. User’s Guide...

  • Page 30: Loading Tubing Into Pump Head

    Pump Channel Pump Head Pump Lever Tubing Retainer 2. On the right (outlet) side of the , press the black PUMP HEAD TUBING RETAINER inward and up until is in the up position (retracted). TUBING RETAINER MP-1000 PourMatic M1224-0050 User’s Guide...

  • Page 31: Installing The Dust Cover

    DUST COVER 2. Insert the into the on the of the DUST COVER CLIPS DUST COVER SLOTS HOUSING PourMatic. Figure 7: Installing Dust Cover Top Down View Dust Cover Slots Dust Cover Clips New Brunswick Scientific Co., Inc. User’s Guide...
  • Page 32 ALWAYS ensure that the GERMICIDAL LAMP sits properly and is flush with the housing. DUST COVER 3. Verify that the sits securely on the and is covering the DUST COVER HOUSING NOZZLE GERMICIDAL LAMP MP-1000 PourMatic M1224-0050 User’s Guide...

  • Page 33: Operation

    You can set the cool time in minute increments and after the cool time has expired, an alarm sounds. SELECT KEY SELECT To disable the alarm, press the after all the dishes are filled. NOTE: You can avoid the cool time alarm, by setting the timer to zero. New Brunswick Scientific Co., Inc. User’s Guide...

  • Page 34: Priming The Pump

    5. Verify that you have entered a SPEED % If the above steps have been performed, you are now ready to prime the . Priming PUMP ensures that the agar is bubble free and flows freely through the SILICONE TUBING MP-1000 PourMatic M1224-0050 User’s Guide...

  • Page 35: Calibration

    Calibration Immediately after priming the , you should calibrate the PourMatic. Calibration is PUMP necessary for the microprocessor to calculate the number of pump revolutions required to pump a specific amount of agar. New Brunswick Scientific Co., Inc. User’s Guide...

  • Page 36: Entering A Dispensing Volume

    CALIBRATE CALIBRATE START / STOP , the PourMatic will re-calibrate. Entering a Dispensing Volume Depending on the size and intended application of your petri dishes, choose the volume of agar to dispense into each dish. MP-1000 PourMatic M1224-0050 User’s Guide...
  • Page 37 DISPENSE VOLUME CALIBRATE DISPENSE VOLUME COOL TIME SPEED % 2. Use the and scroll to the volume of agar you UP/DOWN ARROW KEYS want dispensed into each petri dish. New Brunswick Scientific Co., Inc. User’s Guide...

  • Page 38: Starting The Pourmatic

    4. Verify that the is properly installed and sits flush against the DUST COVER HOUSING of the PourMatic. 5. Verify that you are not in mode. CALIBRATE START 6. Press the START / STOP KEY STOP MP-1000 PourMatic M1224-0050 User’s Guide...

  • Page 39: Unloading Petri Dishes

    DISH RETAINING RODS 3. Remove the dishes by sliding them through the spring loaded DISH RETAINING RODS STATION 7 4. Repeat steps for the remainder of dishes in the column over New Brunswick Scientific Co., Inc. User’s Guide...

  • Page 40: Unloading In Continuous Mode

    DISH RETAINING RODS CAROUSEL BASE STATIONARY PLATFORM 4. Remove the dishes by sliding them through the spring loaded DISH RETAINING RODS STATION 1 5. Repeat steps for the remainder of dishes in the column over MP-1000 PourMatic M1224-0050 User’s Guide...

  • Page 41: Clearing Jams

    SELECT KEY operation. The number of times the is pressed to correct a problem depends on the type of error or jam. New Brunswick Scientific Co., Inc. User’s Guide...

  • Page 42: Carousel Jams (Flashing "Err " Code & Carousel Key )

    4. If the carousel column in the previous step is left empty, rotate the CAROUSEL clockwise to align the trailing dish retaining rod with the dish guide on the stationary platform. TRAILING DISH RETAINING DISH LEADING GUIDE DISH RETAINING STATIONARY PLATFORM MP-1000 PourMatic M1224-0050 User’s Guide...

  • Page 43: Dish Transfer Table Jams (Flashing "Err " Code & Transfer Table Key )

    5. Rotate counterclockwise until the double index mark is aligned with the tip of the dish sensor, and there are no petri dishes before the nozzle or after the elevator station (see following drawing). New Brunswick Scientific Co., Inc. User’s Guide...
  • Page 44 If elevator does not move, press again. 8. Replace the . Verify that the is properly installed and sits DUST COVER DUST COVER flush against the of the PourMatic. HOUSING SELECT 9. Press to resume operation. MP-1000 PourMatic M1224-0050 User’s Guide...

  • Page 45: Dish Errors (Flashing Code <100 In Function Display )

    DISH TRANSFER TABLE 5. Rotate the clockwise until the nearest single index mark is aligned with the tip of the dish sensor to prevent a petri dish from being filled twice (see following drawing). New Brunswick Scientific Co., Inc. User’s Guide...

  • Page 46: Clearing Error Code 50

    3. Visually inspect the DISH TRANSFER TABLE 4. Remove faulty dish components from DISH TRANSFER TABLE 5. Rotate the clockwise until the nearest double index mark is aligned with the tip of the dish sensor (see following drawing). MP-1000 PourMatic M1224-0050 User’s Guide...

  • Page 47: Clearing Other Error Code Numbers

    DISH TRANSFER TABLE 5. Rotate the counterclockwise until the nearest single index mark is aligned with the tip of the dish sensor to prevent a petri dish from being filled twice (see following drawing). New Brunswick Scientific Co., Inc. User’s Guide...

  • Page 48: Elevator Jams (Flashing "Ele" Code)

    To clear an elevator jam: 1. Press to silence alarm. SELECT DUST COVER 2. Remove the 3. Remove dishes in carousel column in elevator station if needed to gain access to jam. 4. Remove dishes from jammed elevator station. MP-1000 PourMatic M1224-0050 User’s Guide...
  • Page 49 SELECT again. DUST COVER DUST COVER 9. Replace . Confirm that the is properly installed and sits flush against the housing of the PourMatic. SELECT 10. Press to resume operation. New Brunswick Scientific Co., Inc. User’s Guide...

  • Page 50: Sensor Errors (Flashing Error Code 102 Or 103)

    5. Replace . Confirm that the is properly installed and sits flush against the housing of the PourMatic. SELECT 6. Press to resume operation. 7. If the error message persists, contact local NBS Service Department. MP-1000 PourMatic M1224-0050 User’s Guide...

  • Page 51: Preventive Maintenance

    , you should remove all parts and wipe thoroughly with a DISH TRANSFER TABLE damp sponge. 4. The Fiber Optic Sensors can also accumulate spilled agar. If this happens, gently wipe the dish sensors with a damp sponge. New Brunswick Scientific Co., Inc. User’s Guide...

  • Page 52: Fuse Replacement

    3. Put the tip of a small flat head screwdriver under the (see FUSE DRAWER LOCK TAB following drawing) and gently pry it open. Figure 13: Fuse Replacement Power Entry Module FUSE HOLDER FUSE DRAWER LOCK TAB MP-1000 PourMatic M1224-0050 User’s Guide...
  • Page 53 6. Gently snap the new fuse into the FUSE HOLDER 7. Slide the back into the and snap into place. FUSE HOLDER POWER ENTRY MODULE 8. Reconnect the to the electrical outlet. POWER CORD New Brunswick Scientific Co., Inc. User’s Guide...

  • Page 54: Troubleshooting

    • Elevator unable to move because of • Follow Procedure for clearing problem with DISH TRANSFER TABLE ELEVATOR ERRORS • There are already 40 petri dishes in elevator station carousel column …continued… MP-1000 PourMatic M1224-0050 User’s Guide...
  • Page 55 Sensor did not scan properly • Error Follow Procedure for clearing • Code 102 SENSOR ERRORS, if message Media may have splashed on dish or 103 persists, contact local NBS sensor Service Department • Dish sensor failed New Brunswick Scientific Co., Inc. User’s Guide...

  • Page 56: Replacement Parts & Accessories

    Bracket Assembly for Domino Ink Jet Printer M1240–1000 14.3 Conversion Kit The PourMatic accommodates either 60mm or 100mm petri dish sizes. Converting to a different dish size requires component changes. To change dish sizes: DUST COVER 1. Remove the MP-1000 PourMatic M1224-0050 User’s Guide...
  • Page 57 11. You must adjust the fiber optic dish sensors when you change dish sizes (see following drawing): • 60 mm size: move L-BRACKET to inner slot location • 100 mm size: move L-BRACKET to outer slot location L-BRACKET 12. Move the into the proper position. New Brunswick Scientific Co., Inc. User’s Guide...
  • Page 58 You must replace properly. The must TRANSFER TABLE LOCATING DOWEL PIN seat properly into the TRANSFER TABLE HUB SLOT 17. Insert the into the center of the and tighten with a BOLT TRANSFER TABLE screwdriver. FLATHEAD (-) MP-1000 PourMatic M1224-0050 User’s Guide...
  • Page 59 24. Verify that the is properly installed and sits flush against the of the PourMatic. CAROUSEL STACKING PLATE 25. After placing 60mm plates in , utilize stainless steel (M1224-9127) on top to ensure smooth operation. New Brunswick Scientific Co., Inc. User’s Guide...

  • Page 60: Drawings

    15.1 Control Schematics Figure 16: Control Schematic (without foot switch) MP-1000 PourMatic M1224-0050 User’s Guide...
  • Page 61 Figure 17: Control Schematic (with foot switch) New Brunswick Scientific Co., Inc. User’s Guide...

  • Page 62: Index Of Drawings

    Realignment for Dish Error Code 50 ONLY Realignment for Dish Errors (EXCEPT Error Code 50) Realignment for Elevator Jam Fuse Replacement Elevator Pad Removal Fiber Optic Sensor Adjustments Control Schematic (without foot switch) Control Schematic (with foot switch) MP-1000 PourMatic M1224-0050 User’s Guide...

  • Page 63: Index

    Dispensing Nozzle Holder · 17 up key · 18 Down Key · 18 Fuse Replacement · 52 Drawings Index of · 62 Dust Cover · 17 installing · 31 Germicidal Lamp · 17, 24 New Brunswick Scientific Co., Inc. User’s Guide...
  • Page 64 · 35 cool time · 33 entering dispensing volume · 36 priming the pump · 34 speed % · 33 WARNING Overview · 9 Symbol for · 5 Warranty · 6 Peristaltic Pump · 17 MP-1000 PourMatic M1224-0050 User’s Guide...

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