Manual Pipetting - Sartorius Picus User Manual

To execute the selected program:
1. Press the operating button to aspirate the liquid (selected volume +
excess) in.
2. Press the operating button again to discard the prime excess.
3. Press the operating button repeatedly untill all aliquots have been
dispensed.
4. To continue repetitive dispensing without discarding the excess volume,
either
a. hold the operating button down and then release it for reaspiration,
OR
b. press the left Softkey for NO and start from the beginning.
5. To discard the excess volume and to empty the tip, press the operating
button.
6. Eject the tip by pressing the electronic tip ejector button.
Additional modes
Excess Adjustment, timed Automated Dispensing and Tracker can be used
in conjunction with Multi-Dispensing.
set the excess volume. Timed Automated Dispensing dispenses the liquid
automatically without the need to press the operating button. Tracker advises
the user in microplate Dispensing by displaying the location for the next
dispensing.
For more information on using additional modes see chapter 3.2.9.
NOTE!
Only one additional mode can be selected at a time.

3.2.4 Manual Pipetting

In Manual Pipetting the piston movement in aspiration and dispensing is
controlled manually turning the adjustment wheel. It is ideal for measuring
reagents, and in applications in which the pipetting speed needs to be
controlled manually.
To select Manual Pipetting mode:
1. Select MENU by pressing the left Softkey.
2. Use the adjustment wheel to select Manual. Confirm the setting by
pressing the operating button or the Softkey for OK.
To edit the parameters (volume, speed):
1. Press the middle Softkey for EDIT or turn the adjustment wheel all the way
to the right. The first parameter to be edited is now highlighted.
2. Turn the adjustment wheel to set the desired value.
3. Confirm the setting by pressing
a. the operating button or the right Softkey for OK, and simultaneously
exit the editing mode
OR
b. the middle Softkey for NEXT, and move on to edit the next, highlighted,
parameter.
4. Repeat steps 2 & 3 for all parameters you wish to edit.
5. To exit the editing mode during programming press the left Softkey for
BACK, and the latest changes are not saved.
To execute the selected program:
1. Press the operating button to activate the aspiration of the liquid.
2. Press the operating button again and hold it down, or turn the adjustment
wheel to the right, to aspirate the liquid. You may influence the aspiration
speed by how far right you turn the adjustment wheel. You may pause the
aspiration by releasing the operating button or the adjustment wheel for
a while.
3. To activate the dispensing of the liquid, turn the adjustment wheel to left.
Keep the adjustment wheel to left, or hold the operating button down as
long as you want to continue dispensing.
12
Excess Adjustment can be used to