Sartorius Proline Plus User Manual
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Sartorius Proline Plus User Manual

Mechanical pipette
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Mechanical
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Summary of Contents for Sartorius Proline Plus

  • Page 1 User Manual Proline Plus ® Mechanical Pipette...

  • Page 2: Table Of Contents

    Table of Contents 1. Intended use ........................3 2. Product description ....................... 3 3. Sartorius pipette tips ..................... 4 4. Unpacking .......................... 4 5. Pipette holder and carousel stand ................. 5 6. Calibration Tool ........................ 5 7. Operating the pipette ....................6 7.1.

  • Page 3: Intended Use

    Tips. The Sartorius Proline Plus product range covers a volume range of ® 0.1 μl to 10,000 μl. It is recommended that Sartorius Optifit Tips or Safetyspace Filter Tips are used with Sartorius pipettes to ensure ® optimum compatibility and performance.

  • Page 4: Sartorius Pipette Tips

    ® air gap Sartorius non-filter tips are available in trays, bulk packages, and space- saving refill systems. Both non-filter tips and trays are autoclavable at 121°C (252°F), 20 min, 1 bar (15 psi). All Single Tray racks and clean Refill Packs are certified RNase, DNase, and endotoxin-free.

  • Page 5: Pipette Holder And Carousel Stand

    5. Pipette holder and carousel stand For convenience and safety, always keep the pipette vertically on its own holder, carousel or linear stand when not in use. Cat.No. Product LH-725630 Carousel Stand for 6 pipettes LH-727640 Pipette Holder for 1 pipette 725620 Linear Stand 6.

  • Page 6: Operating The Pipette

    7.3. Protective tip cone filters The tip cone design of the Sartorius pipettes (>10 µl) allows the use of Safe-Cone Filters in the tip cones. These removable filters prevent liquids and aerosols from entering the pipette and thus, protects the pipette from contamination and damage.

  • Page 7: Pipetting Techniques

    8. Pipetting Techniques Two basic pipetting techniques, forward and reverse pipetting, are associated with Proline Plus pipettes. Forward pipetting is the most ® common used pipetting technique. The technique employs the blow- out function ensuring complete delivery of the liquid. Reverse pipetting is recommended for highly viscous, biological or foaming liquids, or very small volumes of liquid.

  • Page 8: Recommendations For Good Pipetting

    ® If the pipette is used daily it is recommended to clean|decontaminate and check the performance of the pipette every three months. Sartorius also provides complete repair and recalibration service for your pipette including a service report and performance certificate.

  • Page 9: Cleaning The Lower Part Of The Pipette

    If your pipette is in daily use it is recommended to clean|decontaminate and grease the pipette every three months. It is recommended to send the multichannel pipettes to your local Sartorius service representative for cleaning and greasing. To clean and decontaminate the lower parts of...

  • Page 10: Sterilization Of The Pipette

    10.3. Sterilization of The Pipette The entire Proline Plus pipette can be sterilized by steam autoclaving at ® 121°C, (252°F), 1 bar overpressure for 20 minutes. Remove the tip cone filter (if fitted, Chapter 7.3). The single channel pipettes can be autoclaved without special preparations.

  • Page 11: Recalibration

    3 distilled water according to ISO 3696. The calibration is based on ISO 8655-6: Gravimetric test method for volumetric instruments. The pipette's specifications are guaranteed only with genuine Sartorius tips. If you find the pipette to be inaccurate after performance testing, please follow the instructions below: 1.

  • Page 12: Troubleshooting

    ® defects in materials and workmanship. Should your pipette fail to function at any time, please contact your local Sartorius representative. ANY WARRANTY WILL, HOWEVER, BE DEEMED AS VOID IF FAULT IS FOUND TO HAVE BEEN CAUSED BY MALTREATMENT, MISUSE,...

  • Page 13: Pipette Parts And Materials

    14. Pipette Parts and Materials Parts 1. Tip ejector collar: Polypropylene (PP) 2. Tip cone holder: Polyamide (PA) 3. Tip cone complete 5, 10, 20, 25, 50, 100 µl: Polyvinylidene fluoride (PVDF) 200, 250, 500, 1,000, 2,000, 5,000, 10,000 µl: Polyphenylene sulfide (PPS) 4.

  • Page 14: Performance Specifications And Order Information

    15. Performance Specifications and Order Information Proline Plus ® Order Code Channels Volume Range Increment Test Volume Systematic Error Random Error Limit ± Limit (µl) (µl) (µl) (µl) (µl) 728010 0.1 – 3 0.002 0.042 0.024 0.039 0.024 10.0 0.030 0.018 728020 0.5 –...
  • Page 15 Note: The listed systematic and random error values can be achieved only under strictly controlled conditions during type test per ISO 8655. Due to the continuous product development by Sartorius, the systematic and random error values are subject to change without prior notice.
  • Page 16 Bohemia, NY 11716 Phone +358 9 755 951 37079 Goettingen Phone +1 631 254 4249 Phone +49 551 308 0 Toll-free +1 800 635 2906 Specifications subject to change without notice. Copyright Sartorius Biohit Liquid Handling Oy. Status: 09 | 2021...

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