Introduction To Flow Cytometry; Flow Cuvette And Light Signals - SYSMEX CyFlow Ploidy Analyser Instrument Operating Manual

Instrument Operating Manual
2.2

Introduction to Flow Cytometry

The term Flow Cytometry describes a technique for the analysis of cells – or particles – in
aqueous suspension. Cells or other biological particles pass in a single cell stream
through a measuring apparatus. In the most common scenario, one or more lasers
interrogate each particle and, at a minimum, the system measures the degree and
direction of scattered light – indicators of the particle's size, shape and structure. If
particles have been stained with one or more fluorescent dyes – known as fluorochromes
– the light source excites these dyes to provide additional biological information about
each particle, such as the presence of specific cellular compounds. The unique
advantage of Flow Cytometry is that it can rapidly and quantitatively measure multiple
simultaneous parameters on individual live cells.
2.3

Flow Cuvette and Light Signals

Flow Cuvette
The main element of the CyFlow
cuvette. This is made of quartz glass which contains a capillary with a diameter of
200 x 350 µm.
Fluidics
The fluid system of a flow cytometer is used to transport particles from a random
three dimensional sample suspension to an orderly stream of particles travelling
past one or more illuminating beams. The fluidic system uses air pressure
regulation to ensure stable operation and consists of a sheath fluid line and a
sample line feeding into the flow cell. As the sample enters the flow cell chamber,
the outer, sheath fluid flow hydro-dynamically focuses the sample flow into a
narrow core region within the jet and presents a single file of particles to excitation
sources. This geometry provides increased positioning accuracy at the laser
interrogation point for consistent excitation irradiance and greatly reduced particle
blockage of the flow cell.
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CyFlow® Ploidy Analyser Rev-03_2104-10-22
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Ploidy Analyser Flow Cytometer is a flow
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