Table Of Contents; Table Of Content - SYSMEX CyFlow Ploidy Analyser Instrument Operating Manual

Instrument Operating Manual

Table of content

Table of content ................................................................................................................ 3
1. Introduction .......................................................................................................... 5
2. Presentation ......................................................................................................... 6
2.1 Basics .......................................................................................................... 6
What are topics covered by this manual? ................................................................... 6
What other manuals are available? ............................................................................ 6
What should I know before operating the CyFlow
2.2 Introduction to Flow Cytometry ...................................................................... 7
2.3 Flow Cuvette and Light Signals ...................................................................... 7
Optical Standard Setup - Parameters and Filters .......................................... 8
2.4
2.5 In flow cytometry, what is... .......................................................................... 10
... a parameter? ....................................................................................................... 10
... a histogram channel? .......................................................................................... 10
... the count in a histogram? .................................................................................... 10
... a peak?
... background in a histogram? ................................................................................ 10
3. Instrument starting procedure ............................................................................. 11
3.1 Sheath fluid level control and/or refill ........................................................... 11
3.2 Switching on the CyFlow
3.3 User levels ................................................................................................... 13
TM
4. CyView Controls ............................................................................................. 14
CyView™ Instrument Setting Controls ......................................................... 15
4.1
4.1.1
4.1.2
4.1.3
4.1.4
4.1.5
4.1.6
4.1.7
TM
5. CyView Instrument/Measurement Settings ...................................................... 23
5.1 Priming / Initialization process ...................................................................... 23
5.2 Work process ............................................................................................... 25
5.3 Shut down process ...................................................................................... 25
5.4 Intermediate cleaning process ..................................................................... 26
5.5 Sheath and Waste bottle .............................................................................. 27
6. Measurement parameters .................................................................................. 27
Sysmex Partec GmbH
Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com ·
........................................................................................................ 10
®
Ploidy Analyser ................................................... 11
1D/2D PLOTS- display options ....................................................... 15
RESULTS ....................................................................................... 16
REGIONS ....................................................................................... 17
GENERAL - properties of the instrument ......................................... 18
ACQUISITION - instrument control ................................................. 18
MEASURE - definition of measure modes ...................................... 19
Measure modes .............................................................................. 20
CyFlow® Ploidy Analyser Rev-03_2104-10-22
®
Ploidy Analyser? ......................... 6
www.sysmex-partec.com
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