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[ CARE AND USE MANUAL ]
ACQUITY APC Columns
CONTENTS
I.
INTRODUCTION
II.
III.
IV.
V.
VI.
I. INTRODUCTION
Thank you for choosing an ACQUITY APC™ Column. The
ACQUITY APC Columns are designed to achieve maximum
separation performance when used with the ACQUITY™
Advanced Polymer Chromatography™ (APC™) System. Please
take a few moments to read this manual carefully. Following
the recommendations in this manual will prolong column life
and enhance chromatographic reproducibility.
ACQUITY APC Columns are packed with sub-3-µm ethylene
bridged hybrid (BEH) particles that provide mechanical
strength, packed-bed stability and high separation efficiency.
They deliver superior chromatographic performance for all
polymer classes, including low molecular weight aqueous
and organic soluble polymers, up to a molecular weight of
2,000,000 g/mole (Table 1).
To maximize separation selectivity and performance
ACQUITY APC Columns are available in two bonding
technologies optimized for each solvent class:
ACQUITY APC XT: Packed with a high-coverage trimethyl
silane bonded to a BEH substrate. These columns are
recommended for extended temperature (< 90 °C)
separations in organic solvents.
ACQUITY APC AQ: Feature unbonded BEH substrate.
Recommended for room temperature (< 45 °C) separations
in aqueous solvents.
ACQUITY APC Columns are manufactured in an ISO 9001
facility and are held to narrow specification ranges to ensure
reproducible performance. Every column is individually tested
and a Certificate of Batch Analysis is provided on the attached
eCord™ Intelligent Chip.
1
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Related Manuals for Waters ACQUITY APC XT 45
Summary of Contents for Waters ACQUITY APC XT 45
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Page 1: Table Of Contents
[ CARE AND USE MANUAL ] ACQUITY APC Columns CONTENTS I. INTRODUCTION Thank you for choosing an ACQUITY APC™ Column. The INTRODUCTION ACQUITY APC Columns are designed to achieve maximum GETTING STARTED separation performance when used with the ACQUITY™ a. Preparing the System Advanced Polymer Chromatography™... -
Page 2: Getting Started
Recommended Temperature Pore Size Linear Range Separation Limit pH Range (Å) (µm) (g/mole)* Solvent (°C) ACQUITY APC XT 45 Organic 1-11 200-5,000 ACQUITY APC XT 125 Organic 1-11 1,000-30,000 ACQUITY APC XT 200 Organic 1-11 3,000-70,000 ACQUITY APC XT 450... -
Page 3: Ecord Installation
To maximize ACQUITY APC Column lifetime, pay attention to these guidelines: Figure 2. Parker Ferrule in a Waters Style Endfitting. For best resolution and maximum column life, do not exceed ■ ■... -
Page 4: Calibration
[ CARE AND USE MANUAL ] b. Calibration Whenever replacing a single column of a complete column bank, generate a new calibration curve to ensure the reproducibility of the application. Figure 3 shows typical calibration curves for each column. The calibration curves were obtained with polystyrene standards. Figure 4 shows the expected linear range based on the calibration data. -
Page 5: Minimizing Band Spread
The ACQUITY APC System was designed to minimize band used as the mobile-phase solvent, you would be limited to spreading. Deviation from Waters specified tubing could result a maximum flow rate of 1.0 mL/min. In this case, DMF has a in deterioration of chromatographic performance. -
Page 6: Storage
If the column performance is is permanently attached to the column to assure that the poor after cleaning and regenerating, call your local Waters column’s performance history is maintained in the event that office for additional support. -
Page 7: Column Use Information
Waters, The Science of What’s Possible, Empower, UPLC, Advanced Polymer Chromatography, T: 1 508 478 2000 ACQUITY APC, ACQUITY, APC, and eCord are registered trademarks of Waters Corporation. F: 1 508 872 1990 www.waters.com ©2019 Waters Corporation. Produced in the U.S.A. October 2019 720004667EN Rev. C IH-PDF...