Connecting The Gc Detector - Thermo Scientific Nicolet iS50 GC-IR User Manual

iS50 GC-IR Module

Connecting the GC Detector

16 iS50 GC-IR Module User Guide
NOTICE Pieces of graphite, column or dirt in the transfer line or Tee union may
enter the flow cell and block the infrared beam. Keep the Tee union and transfer lines
clean and free of debris.
5. Feed the column through the Tee union and a few millimeters into the transfer line.
NOTICE Do not attempt to feed the wide-bore column through to the flow cell. The
column may become lodged in the Tee and cause blockage of the transfer line.
6. Use a 5/16-in and a 1/4-in wrench to tighten the column nut on the Tee union one-half
turn past finger-tight.
Related Topics
• Installing a Standard Capillary GC Column
• Connecting the Transfer and Make-up Gas Lines
• Connecting the GC Detector
You can connect the GC column to any detector installed on the GC by redirecting a portion
of the column effluent through a splitter. Although this is not required for GC-IR operation,
it allows you to monitor the GC chromatography during GC-IR experiments to make sure
you are getting the expected separation. You may omit this step if desired and connect the
GC column directly to the Tee union as described in the previous sections.
To connect the column to the GC detector, you will need a 10:1 splitter kit (see our local sales
or service representative to order the correct splitter). The kit includes a splitter body, extra
ferrules and two lengths of capillary tubing that are joined at one end by a special ferrule. The
internal diameter of the slightly darker tubing is 10 times larger than that of the lighter one,
which produces the 10:1 split ratio. In the package, the smaller diameter tubing is coiled more
tightly than the larger diameter tubing. Before you remove the coils from the package, make
sure you mark one of the coils so you can tell them apart during assembly.
The GC column attaches to the short end of the splitter (see the diagram below). The joined
end of the two lengths of capillary tubing connects to the long end of the splitter. The larger
diameter tubing from the splitter connects through the Tee union and transfer line to the flow
cell. The smaller diameter tubing connects to the GC detector.
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