3DHISTECH Pannoramic SCAN II User Manual page 78

Pannoramic SCAN II 2.2.0 User's Guide
Pre-scan + high-resolution – creates a High-Resolution fast FL preview image of every
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FOV within the selected area (in single layer DAPI, with an illumination time of 20ms) that
is displayed in the Profile Wizard window. Scanning process is initiated with a simple Pre-
scan, the located sample with auto threshold is selected, and finally, a Pre-scan in high-
resolution is launched.
Thresholding
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The manually set threshold value is applied during scanning. The preview of the loaded slide
is displayed in the Preview window. The set value has effect on the preview image. If
threshold value is modified in the Microscope Controller preview window, that value is
automatically applied in the profile in the Profile Settings panel.
Specimen threshold
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Manually set value: This sets the sensitivity of the specimen detection. To set the desired
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threshold value manually, change the setting in the Specimen threshold level selection
box in the range of 0-255. The default value is 212. Smaller values result in a more
sensitive scanning, meaning that more areas will be digitized, however, this may also
include specks or other imperfections of the slide. Greater values result in a less sensitive
scanning, but it is possible that some of the smaller specimen areas will not be digitized.
Auto Threshold
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By clicking
Make sure that the detection result is acceptable for you. Otherwise, set
the threshold value manually or append missing areas with the help of
appropriate functions in the Preview window.
Use marker with threshold
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If this option is selected, only the area encircled with a marker pen will be scanned. If the
specimen cannot be identified for digitization, (for example, contrast is weak), encircling
the specimen with a black marker pen can be of help before running the detection. You
can define the threshold level by changing the threshold value in the Use marker with
threshold selection box in the range of 0-255. This sets the sensitivity of the specimen
detection inside the marker area.
To set the desired threshold value manually, change the setting in the Specimen
threshold selection box in the range of 0-255. The default value is 105.
January 24, 2020 - Rev. 2
the specimen will automatically be detected in the preview image.
Caution!
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5.2.1 Profile Settings panel
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