4
Using the Pump
Best Practices
Contaminated seal wash solvent
C AU T I O N
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How to deal with solvents
• Use clean bottles only.
• Exchange water-based solvents daily.
• Select solvent volume to be used up within 1 – 2 days.
• Use only HPLC-grade solvents and water filtered through 0.2 μm filters.
• Label bottles correctly with bottle content, and filling date / expiry date.
• Use solvent inlet filters.
• Reduce risk of algae growth: use brown bottles for aqueous solvents, avoid
Select channels for Multi-Channel Gradient Valve (MCGV)
• Use lower channels (A and/or D) for buffer solutions.
• Regularly flush all MCGV channels with water to remove possible salt
• Check compatibility of buffers and organic solvents to avoid precipitation.
Optional Inline Filter
The pump can be equipped with an additional inline filter (Inline Filter
Assembly (5067-5407)) with a nominal filter pore size of 0.3 μL.
Advantages of the inline filter:
• Very small internal volume
• Specified for working at high pressures
• Possibility of back-flushing the filter
56
Do not recycle seal wash solvent to avoid contamination.
Weekly exchange seal wash solvent.
direct sunlight.
deposits.
Agilent 1290 Infinity Binary Pump User Manual