Troubleshooting - CORNING Lambda Plus Instruction Manual

LAMBDA PLUS LB-04-2010
Recalibration conditions:
Ambient temperature, the temperature of the pipettor,
tips and liquid should be within the range 20-25°C and
stabilized during weighing within ±0.5°C.
• Measurements should be conducted using distilled
water
• Balance sensitivity should be suitable for the volume to
be measured
Recalibration procedure:
• Set the dose volume depending on the pipettor volume
according to the following table:
Range
Preset
of the pipettor
Model volume
volumes
[μL]
2 0.1 - 2
10 0.5 - 10
20 2 - 20
100 10 - 100
200 20 - 200
1000 100 - 1000
• Perform 5 aspirations, weigh each one and calculate
the average value of the aspirations
• Calculate average aspirated volume in μL multiplying
the average aspiration amount [mg] by the distilled
water density coefficient [μL/mg], which depends on
temperature and pressure according to the following
table:
Temperature [°C]
20
21
22
23
24
25
If the average aspirated volume exceeds the permissible
value, the following should be done:
• Remove the pipetting pushbutton (Fig. 4A),
Warning: The pipetting pushbutton consists of 2 parts:
the lower volume-setting section (Fig. 1A2) and the
upperbutton (Fig. 1A1). After removal of the pushbutton,
both parts will separate.
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Volume change
Δ Δ V for full turn
Permissible
volumes of the
[μL] [μL] calibration key [μL]
(24 increments)
0.2 0.176 - 0.224 0.06
0.5 0.48 - 0.52 0.33
2 1.94 - 2.06 0.63
10 9.84 - 10.16 2.50
20 19.76 - 20.24 6.30
100 98.4 - 101.6 25.00
Pressure [kPa]
95.0 101.3 105.0
1.0028 1.0029 1.0029
1.0030 1.0031 1.0031
1.0032 1.0033 1.0033
1.0034 1.0035 1.0036
1.0037 1.0038 1.0038
1.0039 1.0040 1.0040
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• Holding the volume setting knob to protect it against
rotation, insert the calibration key into the cuts of the
calibration screw (Fig. 4B),
• Turn the key clockwise to reduce the aspirated volume,
or counterclockwise to increase the volume. One full
turn of the calibration key changes the pipettor aspira-
tion volume by the amount given in the table (Fig. 4C),
• Take out the key and fix the pipetting pushbutton (Fig. 4D).
The pipetting pushbutton should be fixed by placing the
lower section on the arbor first (Fig. 1A2) and then the
button (Fig. 1A1).
Determine the average aspirated volume. The average
volume should be within the permissible range shown in
the table. If the volume exceeds the values stated, the
recalibration procedure should be repeated.
When pipetting liquids with physical properties considerably
different from those of water, follow the rules as
described in section 5.

9 - TROUBLESHOOTING

If you notice an improper pipettor operation, identify the
cause and eliminate the fault. To do this, follow the
instructions in the sequence provided. Replacement of
parts should be required only occasionally and should
not occur under normal pipettor use.
Droplets of liquid remain in the pipet tip.
Possible cause
Action
Possible cause
Action
Droplets of air appear in the liquid aspirated into the tip.
Possible cause
Action
Possible cause
Action
Possible cause
The tip is emptied too fast.
Decrease the speed of pressing the
pipettor pushbutton.
The tip wettability has increased due
to extensive use.
Replace the tip with a new one.
The pipet tip immersion is too shallow.
Immerse the tip deeper according to
the instructions.
The pipet tip is incorrectly pressed
onto the pipettor shaft.
Press the pipet tip firmly.
The tip is damaged or worn out due
to extensive use.
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