Corning ® Pipet Tips; Recommendations; Recalibration - CORNING Lambda Plus Instruction Manual

LAMBDA PLUS LB-04-2010
®
6 - CORNING PIPET TIPS
Corning tips are made from high performance polypropy-
lene and their quality guarantees the precision and accu-
racy associated with the Lambda
pipettor. Strict control is maintained throughout the
manufacturing process to ensure the highest quality.
The accuracy and precision figures for the Lambda
single-channel pipettors are only guaranteed when
Corning tips are used. The use of inferior quality tips will
seriously degrade the performance of the Lambda
single-channel pipettor.
Pipet tips used for volumes between 0.1 μL and 10 μL
are used with Model 2 and 10.
Pipet tips used for volumes between 1 μL and 200 μL are
used with Models 20, 100 and 200.
Pipet tips used for volumes between 100 μL and 1000 μL
are used with Model 1000.

7 - RECOMMENDATIONS

Observing the following recommendations will ensure
maximum possible accuracy and precision of liquid
sampling.
• Make sure to operate the Lambda
nel pipettor slowly and smoothly.
• The depth of immersion in the sample liquid should be
the minimum necessary and should remain constant
during aspiration.
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• The Lambda Plus single-channel pipettor should be
held in a vertical position.
• Change the tip when volume setting is changed or
when a different liquid is to be aspirated.
• Change the tip if a droplet remains on the end of the tip
from the previous pipetting operation.
• Each new tip should be pre-rinsed with the liquid to be
pipetted.
• Liquid should never enter the Lambda
channel pipettor shaft. To prevent this:
- Press and release the pushbutton slowly and smoothly.
- Never turn the pipettor upside down.
- Never lay the pipettor on its side when there is liquid
in the tip.
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• Never force the volume setting beyond its recommended
limits.
• When pipetting liquids with temperatures different from
the ambient temperature, it is recommended to pre-rinse
the tip several times before use.
• Do not pipettor liquids with temperatures above 70°C.
• When pipetting acids or corrosive solutions which emit
vapors, it is recommended to disassemble the shaft
and rinse the piston and seal with distilled water after
finishing the pipetting operation.

8 - RECALIBRATION

™
Lambda Plus single-channel pipettors are calibrated
gravimetrically, using Corning tips and distilled water, at a
temperature of 20±1°C, according to EN ISO 8655 stan-
dard.
If during pipettor operation you find that the accuracy
error (the difference between the real aspirated volume
and the preset volume) exceeds the permissible value
given in the table in section 1, the pipettor recalibration
procedure should be carried out.
Before starting the recalibration it is necessary to check
whether the following requirements have been met during
error determination:
• the ambient temperature, and the temperature of the
pipettor, tips and water was identical
• the density of the liquid used is close to that of distilled
water
• a balance with appropriate sensitivity has been used
Volume checked [μL]
0.1 - 10
10 - 100
> 100
• mg/μL conversion factor has been taken into account
• the requirements shown in sections 3 and 7 have been
met
If the above conditions are satisfied and the accuracy
error for selected volume given in section 1 exceeds the
permissible value, the pipettor recalibration procedure
should be carried out.
The recalibration can be performed within one full turn of
the key to the right or to the left only.
Balance sensitivity [mg]
≤ 0.001
≤ 0.01
≤ 0.1
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