Setting The Volume - CORNING Lambda Plus Instruction Manual

LAMBDA PLUS LB-04-2010
Volume
Model
[μL]
2
Max
Min
10
Max 10.0
Min
20
Max
Min
100
Max 100
Min
100
200
Max 200
Min 100
1000 500
Max 1000
The accuracy and precision are obtained gravimetrically
using Corning tips, performing at least 10 measurements
of distilled water at a temperature of 20±1°C, according
to EN ISO 8655 standard.
The design enables the user to recalibrate the pipettor
themselves according to the rules described in section 8.

2 - SETTING THE VOLUME

Setting the volume of the aspirated liquid can be done
either using the pipetting pushbutton (Fig. 1A2) or by the
adjustment knob (Fig. 1B). The volume display shown by
the counter has three digits. These should be read from
top to bottom. The lowest counter dial shows a scale
which allows volume setting in the minimum
graduation range. Examples of the black and red digits:
Pipettor 2
Red figures at the bottom = 1/100 μL
Increment = 0.002 μL
Pipettor 10, 20
Red figures at the bottom = 1/10 μL
Increment = 0.02 μL
3
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Accuracy Precision
[%] [%]
0.2 ± 12.0 ± 6.0
1.0 ± 2.7 ± 1.3
2.0 ± 1.5 ± 0.7
0.5 ± 4.0 ± 2.8
5.0 ± 1.0 ± 0.6
± 0.5 ± 0.4
2 ± 3.0 ± 1.5
10 ± 1.0 ± 0.5
20 ± 0.8 ± 0.3
10 ± 1.6 ± 0.80
50 ± 0.8 ± 0.24
± 0.8 ± 0.20
20 ± 1.2 ± 0.60
± 0.8 ± 0.25
± 0.6 ± 0.20
± 1.6 ± 0.40
± 0.7 ± 0.20
± 0.6 ± 0.15
2
10 20
21-04-10 15:31 Page 8
Pipettor 100, 200
Black figures only = 1 μL
Increment = 0.2 μL
Pipettor 1000
Red figures at the top - mL
Increment =
To set the required volume, use the lower section of the
pipetting pushbutton (Fig. 1A2) or the adjustment knob
(Fig. 1B), to increase or reduce the volume. The volume
adjustment can only be performed when the locking ring is
set in the lower position (Fig.5A).
When the desired volume is set, the locking ring should be
moved to the upper position (Fig. 5B). All volume setting
procedures should end in the the reduction of volume
setting from a position just above the desired level to that
level. As further examples:
• When moving from a high volume setting to a lower
value, the pushbutton or adjustment knob should be
rotated at normal speed, clockwise, to take the setting
just above the desired level. It should then be further
rotated very slowly till the desired volume is shown on
the digital volume display.
• In the event that you overshoot the desired setting,
repeat the procedure by increasing the volume to
a position just above the desired volume and then once
again, slowly adjust clockwise to the desired level.
• When moving from a low volume setting to a higher
value, the pushbutton or adjustment knob should be
rotated at normal speed, counterclockwise, to take the
setting to a position just above the desired level. Then
slowly reverse the rotation clockwise and adjust back
until the desired volume is shown on the digital display.
• Once again, in the event that you overshoot the desired
setting, repeat the procedure by increasing the volume
to a position just above the desired volume and then
slowly adjust clockwise to the desired level.
• Following volume adjustment, move the locking ring to
the upper position, to lock the knob and prevent
accidental volume change.
100 200
1000
2 μL
4