Table of Contents
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Manual Peptide Synthesizer
Installation and Operation Instructions
Contents
Discover Bio
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Rev. 2
August 2017
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Table of Contents
Related Manuals for CEM Discover Bio
Summary of Contents for CEM Discover Bio
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Page 1: Table Of Contents
Discover Bio Manual Peptide Synthesizer Installation and Operation Instructions Contents Introduction to the Discover Bio Performing a Cleavage Using the 4 mL Vessel for Smaller Scale 15 System Requirements Hardware Assembly and Setup Connecting the 4 mL Vessel Maintenance Procedures... -
Page 2: Introduction To The Discover Bio
The Discover Bio Manual Microwave Peptide Synthesizer is the newest manual microwave-enhanced peptide synthesis system. Built on CEM’s flexible Discover microwave platform, the Discover Bio allows the rapid and efficient synthesis of peptides on scales from 0.05 to 0.5 mmol thanks to the efficient washing station and CEM’s patented circular microwave cavity. -
Page 3: System Requirements
Electrical Requirements The Discover Bio requires electrical power of 120 VAC (60 Hz, 1.7 A) (or 240 VAC [50 Hz, 1.7 A] where applicable). Specific power requirements (120 VAC vs. 240 VAC) can be found on the nameplate affixed to the rear of the Discover Bio Microwave Reactor and on the rear of the Discover Bio Wash Station. -
Page 4: Hardware Assembly And Setup
3.3. Connect the other end of the bottle tubing to the rear of the Wash Station. The tube labeled “LIQ” should be connected to position 5. The unlabeled tube should be connected to position Discover Bio Manual Microwave Peptide Synthesizer ™... - Page 5 2.3. Using a yellow ferrule and nut, connect the other end of the tubing to position 7 on the rear of the Wash Station. Discover Bio Manual Microwave Peptide Synthesizer ™ 600423 • Revision 2 • August 2017...
- Page 6 4.2. Using a yellow ferrule and nut, connect the other end of the tubing to position 9 on the rear of the Wash Station 5. Connect the RV Drain line. Discover Bio Manual Microwave Peptide Synthesizer ™ 600423 • Revision 2 • August 2017...
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Page 7: Discover Setup
9. Ensure the GF number is correctly entered, and press ENTER to save the calibration value in the software. 10. Press HOME to return to the Home screen. Discover Bio Manual Microwave Peptide Synthesizer ™ 600423 • Revision 2 • August 2017... -
Page 8: General Instrument Operation
) to scroll to the desired method name, then press ENTER to load the method. 3. The method name should be displayed on the Home screen, along with the programmed parameters. Discover Bio Manual Microwave Peptide Synthesizer ™ 600423 • Revision 2 • August 2017... -
Page 9: Recommended Synthesis Parameters
The goal is to reach the maximum temperature in 75 seconds for Deprotection and Coupling, and in 120 seconds for Cleavage. WARNING The Discover Bio reaction vessels should not be operated above 75 ºC. Recommended Reagents for 0.1 mmol Scale Activator Base Solution Prepare a solution of ~0.3 M DIEA in DMF. - Page 10 0.194 0.190 Fmoc-Pro-OH 337.4 0.169 0.190 Fmoc-Ser(tBu)-OH 383.4 0.192 0.190 Fmoc-Thr(tBu)-OH 397.5 0.199 0.190 Fmoc-Trp(Boc)-OH 526.6 0.263 0.190 Fmoc-Tyr(tBu)-OH 459.6 0.230 0.190 Fmoc-Val-OH 339.4 0.170 0.190 Discover Bio Manual Microwave Peptide Synthesizer ™ 600423 • Revision 2 • August 2017...
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Page 11: General Discover Bio Methods
General Discover Bio Methods Swelling the Resin 1. Set both knobs to “Stop”. NOTE It does not matter which “Stop” position is used on the right knob. 2. Turn on the nitrogen source, then turn on the nitrogen to the system by setting the nitrogen control knob to pressure (the knob should be pointing down) and the vacuum pump. -
Page 12: Performing A Deprotection
Play/Pause key to begin the method. WARNING The Discover Bio reaction vessels should not be operated above 75 ºC. 6. When the microwave method is finished, remove the reaction vessel from the Discover cavity and place it in the vessel holder. Set the left knob to “N2 Purge (Wash)” the right knob to “Waste”. -
Page 13: Performing A Cleavage
Play/Pause key to begin the method. WARNING The Discover Bio reaction vessels should not be operated above 75 ºC. 9. When the microwave method is finished, remove the reaction vessel from the Discover cavity and place it in the vessel holder. Set the right knob to “Waste” to drain the reaction vessel. - Page 14 18. Set both knobs to “Stop.” Turn off the nitrogen source. 19. Remove the solvent bottle, then connect a solvent bottle of wash solvent. 20. Turn on the nitrogen source. Discover Bio Manual Microwave Peptide Synthesizer ™ 600423 • Revision 2 • August 2017...
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Page 15: Using The 4 Ml Vessel For Smaller Scale
Secure the thermowell with the nut. 2. Connect the RV Vent line to the side port of the reaction vessel top. 3. Connect the RV Drain line to the port on the bottom of the vessel. Discover Bio Manual Microwave Peptide Synthesizer ™... -
Page 16: Maintenance Procedures
Replacement of Reaction Vessel Frit 1. Remove the drain line from the bottom of the reaction vessel. 2. Open the reaction vessel by pressing up into the reaction vessel top and rotating. Discover Bio Manual Microwave Peptide Synthesizer ™ 600423 • Revision 2 • August 2017... - Page 17 6. Screw the vessel body into the bottom of the reaction vessel. Close the reaction vessel by pressing up into the reaction vessel top and turning counterclockwise. Connect the drain line to the bottom of the reaction vessel. Discover Bio Manual Microwave Peptide Synthesizer ™...
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Page 18: Spare Parts And Accessories
Spare Parts and Accessories System Product Part Number Discover Bio System 925496 Includes the following: Discover Bio Microwave Reactor (select one) 120 v (60 Hz) Power Supply 909450 240 v (50 Hz) Power Supply 909455 Power Cord (varies by country) - Page 19 Limited Warranty What Is Covered: CEM Corporation warrants that the instrument will be free of any defect in parts or workmanship and will, at its option, replace or repair any defective part (excluding consumables) or instrument. For How Long: This warranty remains in effect for 365 days from date of delivery to the original purchaser.
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Page 20: 600423 Rev
CEM S.R.L. Via Dell’ Artigianato, 6/8 24055 Cologno al Serio (Bg) Tel: (39) 35-896224 Fax: (39) 35-891661 Email: info.srl@cem.com www.cemmicroonde.com Japan CEM has been an ISO-certified CEM Japan K.K. facility since 1994. 2-18-10 Takanawa Minato-ku, Tokyo 108-0074 Tel: +81-3-5793-8542 Fax: +81-3-5793-8543 Email: info@cemjapan.jp...