Performing A Deprotection; Performing A Coupling - CEM Discover Bio Installation And Operation Instructions Manual

5. When the desired volume of solvent has been added to the vessel, set the left knob to "N
(Wash)" to purge any solvent remaining in the lines. When all solvent has been purged into the
vessel, set the left knob to "Stop".
Purging the line will add an additional 1-2 mL of solvent to the reaction vessel.
6. Allow the resin to swell for approximately 15 minutes. Optionally, the resin can be bubbled during
swelling by setting the right knob to "N
7. After the resin has swollen, drain the solvent from the reaction vessel by setting the left knob to
"N Purge (Wash)" the right knob to "Waste". Once all the solvent has drained from the vessel, set
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the right knob to "Stop".

Performing a Deprotection

1. Set the left knob to "Deprotect" to begin adding deprotection solution to the reaction vessel.
2. When the desired volume of deprotection solution has been added to the vessel, set the left knob
to "N2 Purge (Deprotect)" to purge any remaining deprotection solution in the lines. When all
reagent has been purged into the vessel, set the left knob to "Stop".
Purging the line will add an additional 1-2 mL of deprotection solution to the reaction vessel.
3. Set the right knob to "N2 Bubbling".
4. Ensure the fiber-optic probe is fully inserted into the thermowell.
5. Insert the reaction vessel into the Discover cavity. Load the Deprotection microwave method, and
press the Play/Pause key to begin the method.
6. When the microwave method is finished, remove the reaction vessel from the Discover cavity and
place it in the vessel holder. Set the left knob to "N2 Purge (Wash)" the right knob to "Waste".
7. When all the solvent has drained, set the right knob to "Stop". Set the left knob to "Wash Solvent"
and add approximately 7 mL of solvent to the vessel, then set the left knob to "Stop". Optionally,
to bubble the resin for a more efficient wash, turn the right knob to "N2 Bubbling" and allow the
resin to bubble for five seconds.
8. Set the left knob to "N2 Purge (Wash)", then set the right knob to "Waste" to drain the reaction
vessel. Set both knobs to "Stop".
9. Repeat steps 7 and 8 two more times.

Performing a Coupling

1. Set the left knob to "Add" and the right knob to "Stop".
2. Dissolve the appropriate amino acid, activator, and activator base in a total volume of
approximately 4 mL.
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The Discover Bio reaction vessels should not be operated above 75 ºC.
Discover Bio
NOTE
Bubbling" after the solvent has been added.
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NOTE
WARNING
Manual Microwave Peptide Synthesizer
™
600423 • Revision 2 • August 2017
Purge
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