CEM Discover SPS Quick Reference Manual

Microwave-Enhanced
Science
PO Box 200
Matthews, NC 28105
800-726-3331
704-821-7015
www.cem.com
Suggested Parameters for Fmoc Solid Phase Synthesis
NOTE: All values for Power are suggested, and should be adjusted depending on sample size. The goal is to reach the
maximum temperature in 75 seconds for Deprotection and Coupling, and in 120 seconds for Cleavage. The Cleavage
temperature should NEVER exceed 43 °C.
Fmoc Deprotection:
Power = 20 W
Maximum Temperature = 75 °C
Time = 3:00
Delta Temperature = 5 °C
1. Press the Open Folder key.
2. Select "New Method".
3. Press the right arrow key until "Mode = SPS". Press ENTER.
4. Set Power, then press ENTER.
5. Set Maximum Temperature, then press ENTER.
6. Set Method Run Time, then press ENTER.
7. Set Delta Temperature to 5 °C, then press ENTER.
Note: HOBt is optional, but is strongly recommended for
sequences susceptible to aspartimide formation.
Discover SPS
Quick Reference Guide
Coupling:
Power = 20 W
Maximum Temperature = 75 °C
Time = 5:00
Delta Temperature = 5 °C
Method Programming
Reagents for Fmoc Solid Phase Synthesis
Note: Do not add
Activator Base Solution
to Amino Acid/Activator
until immediately before
coupling.
TFA Cleavage:
Power = 20 W
Maximum Temperature = 38 °C
Time = 30:00
Delta Temperature = 5 °C
8. Set Stirring to "OFF", then press ENTER. (NOTE: Magnetic
stirring is not recommended with solid phase synthesis resins.)
9. Set Cooling to "OFF", then press ENTER.
10. Set "Next Stage = (N)", then press ENTER.
11. Set "Save Method = (Y)", then press ENTER.
12. Create a method name using the arrow keys, highlight "Exit",
and press ENTER. The method is now saved in the software.