Programming The Em Cpd300 Procedure; Filling The Sample Chamber With Exchange Fluid; Inserting Specimen Into Sample Container - Leica EM CPD300 Operating Manual

7.3

Programming the EM CPD300 procedure

Before placing the sample into the pressure chamber, program the EM CPD300
protocol. Switch to the program screen panel and follow the instructions under
Program Panel (see page 17-25). Do not forget adjustment of the pressure threshold
for the bottle empty function (see page 38-39).
7.4

Filling the Sample Chamber with exchange fluid

Open the screw-on cover of the sample chamber by turning cover counter clockwise.
Before filling the sample chamber with exchange fluid, place the magnetic stirrer rod
in bottom of the chamber.
Fill exchange fluid into the sample chamber until the stirrer is covered.
Then fill the sample container with exchange fluid to such a level that the sample
holder and specimen will be completely submerged when inserted.
7.5

Inserting Specimen into Sample Container

Open lid and transfer prepared specimen into a suitable sample holder. The sample
holder must be filled with exchange fluid prior to transfer.
Insert the sample holder into the sample container and fill sample container with
exchange fluid until the sample holder is covered. Transfer the sample container into
the sample chamber and check if sample holder is still covered with exchange fluid.
Adjust container volume if required with fillers or additional sample holders.
If sample holders with the volume of 33 – 50% are used, more sample holders or
fillers can be added to the pressure chamber (up to 100%).
Fully open the shut-off valve of the CO
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Sample Container with quick release
pin
bottle.
2