Chapter 1. Qx200 Droplet Digital Pcr System; Introduction - BIO RAD QX200 Instruction Manual

1
QX200 Droplet Digital
PCR System

1.1 Introduction

The QX200 Droplet Digital PCR (ddPCR) System performs accurate and precise
digital PCR. The system consists of two instruments, the QX200 Droplet
Generator and the QX200 Droplet Reader, and their associated consumables.
The QX200 Droplet Generator partitions samples into ~20,000 nanoliter-sized
droplets and, after PCR on a thermal cycler, droplets from each sample are
analyzed individually on the QX200 Droplet Reader. PCR-positive and PCR-
negative droplets are counted to provide absolute quantification of target DNA
in digital form. Alternatively, amplified products can be extracted from droplets
following PCR for downstream applications, such as sequencing or cloning.
The ddPCR System lets you:
Detect rare DNA target copies with unmatched sensitivity
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Determine copy number variation with unrivaled accuracy
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Measure gene expression levels with precision
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Applications and uses include:
Copy number variation
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Rare sequence detection
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Gene expression analysis
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Next-generation sequencing (NGS)
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library quantification
Viral load determination
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Single-cell gene expression analysis
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For instructions on use of the QX200 Droplet Generator, please refer to the QX200 Droplet
Generator Instruction Manual (bulletin 10031907). For information on QX200 AutoDG Droplet
Digital PCR System and the Automated Droplet Generator, please refer to the Automated
Droplet Generator Instruction Manual (bulletin 10043138).
Absolute quantification
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Rare mutant detection
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miRNA analysis
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NGS sample preparation
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GMO detection
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Genome edit detection
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QX200 Droplet Reader and QuantaSoft Software Instruction Manual | 1