BIO RAD QX200 Instruction Manual
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BIO RAD QX200 Instruction Manual

Droplet generator
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QX200
Droplet Generator
™
Instruction Manual
Catalog #186-4002

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Summary of Contents for BIO RAD QX200

  • Page 1: Qx200 Droplet Generator

    QX200 Droplet Generator ™ Instruction Manual Catalog #186-4002...
  • Page 3 Bio-Rad Laboratories. Bio-Rad reserves the right to modify its products and services at any time. This instruction manual is subject to change without notice. Although prepared to ensure accuracy, Bio-Rad assumes no liability for errors, or for any damages resulting from the application or use of this information.
  • Page 4 Alteration of this instrument voids the warranty and safety certification and creates a potential safety hazard. This instrument is intended for laboratory use only. Bio-Rad Laboratories is not responsible for any injury or damage caused by use of this instrument for purposes other than those for which it is intended, or by modifications of the instrument not performed by Bio-Rad Laboratories or an authorized agent.
  • Page 5 Nitrile gloves are made of copolymer and provide protection from chlorinated solvents such as trichloroethylene ■ ■ and tetrachloroethene; they offer protection when working with oils, greases, acids, and caustic substances QX200 Droplet Generator Instruction Manual...
  • Page 6 QX200 Droplet Generator Instruction Manual...

  • Page 7: Table Of Contents

    1.1 Introduction 1.2 QX200 Droplet Generator 1.3 Installation and General Operation Chapter 2 Droplet Generation 2.1 Sample Preparation 2.2 Operation of the QX200 Droplet Generator 2.3 Preparation for PCR 2.4 Subsequent Steps Chapter 3 Specifications and Maintenance 3.1 Specifications 3.2 Maintenance...
  • Page 8 QX200 Droplet Generator Instruction Manual...

  • Page 9: Chapter 1 Qx200 ™ Droplet Generator

    ■ ■ Viral load determination GMO detection ■ ■ ■ ■ This manual covers use of the QX200 droplet generator and preparation for PCR. For information on the QX200 droplet reader, please refer to bulletin 10031906. QX200 Droplet Generator Instruction Manual...

  • Page 10: Qx200 Droplet Generator

    96-well PCR plate and amplified to end point using a standard thermal cycler. When cycling is complete, the plate is loaded into the QX200 droplet reader. The droplet reader sips each sample, singulates the droplets, and streams them in single file past a two-color detector. The detector reads each droplet and determines which contain a target (+) and which do not (–).

  • Page 11: Installation And General Operation

    181-4000 8-cap strips 1.3 Installation and General Operation Connect the QX200 droplet generator to a power source using only the power cord provided. Ensure the ■ ■ ground is reliably connected before plugging in the instrument Leave 10" (5 cm) clear space behind and 5" (2.5 cm) clear to the right and left for proper ventilation ■...
  • Page 12 Chapter 1 QX200 Droplet Generator QX200 Droplet Generator Instruction Manual...

  • Page 13: Chapter 2 Droplet Generation

    Use standard lab precautions to avoid contamination of the reaction mix and ■ ■ sample: wear gloves, work in a clean area (such as a PCR hood), and use clean pipets and low protein binding tubes QX200 Droplet Generator Instruction Manual...

  • Page 14: Operation Of The Qx200 Droplet Generator

    Droplet Generator ™ The QX200 droplet generator prepares droplets for up to eight samples at a time. Droplet generation takes ~2 minutes for each set of eight samples (~30 minutes for a 96-well plate). All 8 sample wells in the DG8 droplet generator cartridge must contain sample (or 1x buffer ■...
  • Page 15 Table 1.2 for PCR supermix and DG oil compatability). Table 2.1. Droplet generator (DG) oil requirements. # Wells Volme of Oil 700 μl 1,820 μl 3,500 μl 6,860 μl Reagent trough and droplet generator oil. QX200 Droplet Generator Instruction Manual...
  • Page 16 Correct placement of the gasket over the cartridge holder. 6. Open the QX200 droplet generator by pressing the button on the green top and place the cartridge holder into the instrument. When the holder is in the correct position, both the power (left light) and holder (middle light) indicator lights are green (see Table 2.2)
  • Page 17 Keep the DG8 cartridge in the holder. Table 2.2. Status indicator lights on the QX200 droplet generator. If the central LED flashes amber, the gasket is not placed on the holder correctly or is missing and no seal was made. If the right LED flashes amber, a process error occurred because the volume is too low in at least one well.

  • Page 18: Preparation For Pcr

    To prevent evaporation and contamination with particulates, cover the plate (for example, with 8-cap strips or the lid from a pipet tip box) as you work. Aspirating droplets from the DG8 cartridge. Dispensing droplets into a 96-well PCR plate. QX200 Droplet Generator Instruction Manual...
  • Page 19 Cover the 96-well plate with one sheet of pierceable foil seal. (The yellow label on the Bio-Rad heat seal bag identifies the sealing surface.) Do not attempt to place the frame over the foil-covered plate. The frame is only for use with other seals.

  • Page 20: Subsequent Steps

    For example, a set of eight wells in a single DG8 cartridge can be generated: four of these will be read after thermal cycling, and four will not be read. Refer to the QX200 Droplet Reader Instruction Manual (bulletin 10031906) for more details.

  • Page 21: Chapter 3 Specifications And Maintenance

    2 (indoor use) Installation category II (external power supply plugs into standard AC receptacle) 5" (12 cm) left and right of machine and 10" (25 cm) Ventilation requirement behind should be unobstructed for proper ventilation QX200 Droplet Generator Instruction Manual...

  • Page 22: Maintenance

    Apply standard MSDS (Material Safety Data Sheet) and OSHA practices when handling and disposing of generated waste. Bio-Rad droplet generation and reader fluids are based on fluorinated hydrocarbon chemistry and should be disposed of in accordance with institutional, state, and local regulations. These nonflammable fluids are inert and have low environmental impact and low toxicity.

  • Page 23: Appendix A Ordering Information

    186-3051 DG8 Cartridge Holder 2x supermix 510-10608 Droplet Reader Plate Holder 186-3024 Droplet PCR Supermix, 5 ml (5 x 1 ml), 2x supermix 186-3025 Droplet PCR Supermix, 25 ml (5 x 5 ml), 2x supermix QX200 Droplet Generator Instruction Manual...
  • Page 24 50 ml (10 x 5 ml), 5,000 x 20 µl reactions 186-3052 ddPCR Buffer Control Kit for Probes, 2 x 4.5 ml bottles, 2x buffer 186-4052 ddPCR Buffer Control Kit for EvaGreen, 2 x 4.5 ml bottles, 2x buffer QX200 Droplet Generator Instruction Manual...
  • Page 28 Life Science Web site www.bio-rad.com USA 800 424 6723 Australia 61 2 9914 2800 Austria 01 877 89 01 Belgium 09 385 55 11 Brazil 55 11 5044 5699 Canada 905 364 3435 China 86 21 6169 8500 Czech Republic 420 241 430 532 Denmark 44 52 10 00 Finland 09 804 22 00...

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