MicroCal VP-ITC User Manual page 71

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Click on the Read Data button, to import your VP-ITC data into the plot window. Find your
way to the data folder and select the file containing your experimental methanol data, (for this
example we will use an example file named methanol.itc, your data folder contains a slightly
different data file named methanol.itc with methanol data collected using your instrument,
but you should be analyzing your experimental data) and click Open.
Origin will automatically plot the raw data, generate a baseline for the raw data, integrate all
peaks, and display the integration results in the Delta H window. This data were generated at
MicroCal on an VP-ITC instrument using 10ul injections of a 2.5% methanol solution. Your
peak sizes may differ a little.
Raw ITC Data Window
Origin automatically plots concentration-normalized areas (kcal per mole of injectant, designated
with an .ndh extension) into the Delta H template whereas what we want to view is the raw data
heats (µcal per injection, designated with a .dh extension).
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Double-click on the layer icon box (a gray box with '1' in it) located in the upper left to open
the layer 1 dialogue box of the Delta H window.
•
Move the methanol_ndh file out of the Layer
Contents (click on the name methanol_ndh then
click on the '<='button) and move the
methanol_ndh file from Available Data to Layer
Contents (click on methanol_dh then click on the
=> button) , and click on OK.
The new data set will be plotted as a line. You may
change this plot symbol by right-clicking anywhere on the plot line and selecting Change to
Scatter from the drop down menu list. Double-click on the numerical tick labels on the Y-axis,
which causes the dialogue box for the Y-axes tick labels to appear. In the Factor box in the upper
Section 4: Experimental Tutorials
61
DeltaH Window