Section 3: Running An Itc Experiment - MicroCal VP-ITC User Manual

Section 3: Running an ITC Experiment

This section provides detailed information about conducting an experiment, from designing,
sample preparation, loading samples, running the experiment and finally cleaning the equipment
after the experiment. New users may find this section too detailed for their immediate needs and
may proceed to the Section 4 tutorials for a 'hands on' approach to learning the instrument
through simple experiments. While performing the tutorials, the user may refer to this section if
further detail is desired about a specific operation. After performing the tutorials, it is advised to
read through this entire section for a thorough understanding of running an ITC experiment.
This section is arranged as follows:
Section 3: Running an ITC Experiment ....................................................................................35
3.1 Designing ITC Experiments ................................................................................................................36
3.2 Using the ThermoVac to Degas Samples ............................................................................................40
3.3 Cell Loading ........................................................................................................................................41
3.4 Loading the Auto-Pipette.....................................................................................................................42
3.5 Choosing The Run Parameters ............................................................................................................45
3.6 Carrying Out Experimental Runs ........................................................................................................46
3.7 Using the ThermoVac for Cleaning the ITC Sample Cell ...................................................................47
3.8 Cleaning Syringes................................................................................................................................49

Section 3: Running an ITC Experiment

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