Methanol Tutorial - MicroCal VP-ITC User Manual

VP-ITC MicroCalorimeter User's Manual
A methanol injection run requires a higher level of precision operation of the VP-ITC instrument
than a water-water run to obtain optimal results. The integrated heats of each injection should be
linear with the probable exception of the first peak.
The recommended procedures for preparation of solutions, degassing, filling the cell and injection
syringe, etc. have been discussed in the previous sections and the following tutorial assumes your
familiarity with those procedures and completion of Section 4.1 Water-water baselines.
Sample Preparation
•
Fill the sample cell with degassed water.
•
Prepare 20 ml of the degassed water in a test tube and add methanol (0.5 ml) to make a 2.5%
methanol solution.
•
Degas the methanol solution for 5 minutes while stirring.
•
Load the methanol solution into the injection syringe of the pipette.
Conducting the Experiment
•
Load the run parameters from the methanol.inj file.
•
Enter a filename. (the name should have no spaces, periods or hyphens and should be less
than 16 characters long).
•
Execute the run and allow the system to complete the experiment.
Analyzing the Data
•
Minimize the VPViewer application by clicking on the Minimize button in the upper right
corner of the window.
•
Open Origin for data analysis, by
selecting Start, from the Taskbar,
then select Programs:MicroCal
Origin:MicroCal Inc. ITC. You
should see the ITC raw data
template.

4.2 Methanol Tutorial

Experimental Procedure
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