Troubleshooting Guide - BIO RAD Aurum Instruction Manual

Table 1. Pressure unit conversions
To convert from inches of mercury (" " Hg) to
millimeters of mercury or torr (mm Hg, torr)
millibar (mbar)
atmospheres (atm)
pounds per square inch (psi)
kilopascals (kPa)
Table 2. Vacuum manifolds and their preparative formats
Vacuum Purification Mini
Aurum Vacuum Manifold •
Promega Vac-Man •
Promega Vac-Man 96
QIAGEN QIAvac 96
QIAGEN QIAvac 24 •
Millipore vacuum manifold
Multiply by:
25.4
33.85
0.03342
0.4912
3.385
Midi Maxi 96
• • •
•
•
•
12
Section 9

Troubleshooting Guide

Problem Possible Cause
Insufficient negative Open vacuum regulator
pressure in vacuum
manifold
Manifold top not seated
properly on base
Purification plate or CAP
not fully seated
Unused CAP luer fittings
not closed completely
caps
Gasket residue preventing Rinse manifold with
even contact between
manifold parts
Lower gasket misaligned
Physical damage to
manifold or CAP (e.g.
chips and cracks)
Purge step in purification
protocol
Excessive noise Purification plate or CAP
during vacuuming not fully seated
Gasket residue preventing Rinse manifold with
even contact between
manifold parts
Possible Solution
Close vacuum regulator
by turning completely
clockwise
Ensure uniform contact
between manifold top
and base
Use A stage for
waste collection
Press down gently on
plate or CAP to seal
Close unused luer
fittings completely with
distilled water
Press gasket back into
groove
Replace vacuum
manifold or CAP
If closure of wells or
columns (e.g. with
sealing tape restores
negative pressure, no
corrective action
needed.
Press down gently on
plate or CAP to seat
distilled water
13