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Summary of Contents for BIO RAD Aurum
- Page 1 ™ Aurum Vacuum Manifold Bio-Rad Laboratories 2000 Alfred Nobel Dr. Hercules, CA USA 94547 (510) 741-1000 Instruction Manual 1-800-424-6723 For technical service, call your local Bio-Rad office, or in the US, call 1-800-4BIORAD (1-800-424-6723) 4110115 Rev A...
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Page 2: Table Of Contents
Care and Maintenance ....2 Section 4 The Aurum Vacuum Manifold ....3 Section 5 Instrument Setup and Use for 96-well Plates . -
Page 3: Introduction
Section 1 Introduction The Aurum vacuum manifold and column adaptor plate (CAP), comprise a patent-pending system which allows a single vacuum manifold to be used with either 96-well plates or columns with luer ends. Separate manifolds are no longer required for different preparative formats; the Aurum vacuum manifold and CAP ensure fast, high-quality sample preparation in both formats, while maintaining the simplicity of handling associated with vacuum processing. -
Page 4: Care And Maintenance
Section 4 The Aurum Vacuum Manifold The Aurum vacuum manifold is designed to accommodate the 96-well plates used in Bio-Rad's kits, such as the Aurum plasmid 96 kit. The manifold may Manifold base Vacuum regulator Manifold top also be compatible with other manufacturers' kits. For column processing, the column adaptor plate is required. -
Page 5: Instrument Setup And Use For 96-Well Plates
Vacuum elution conditions When eluting from the purification plate, remove the A stage and waste collection tray from the Aurum vacuum manifold. Place the B stage on the manifold base, with the etched letter B facing up. Place a clean 96-well Fig. -
Page 6: The Column Adaptor Plate (Cap)
Tubing provided is 4 ft long and must be cut into appropriate pieces before proceeding. columns, or 9 Aurum maxiprep columns* can be accommodated on the CAP without the need for connectors or other manifold accessories. The CAP will Vacuum Setup Conditions also accommodate other columns with luer ends. -
Page 7: Instrument Setup And Use For The Column Adaptor Plate
Vacuum set-up conditions (please see Figure 3, page 4). 11. After finishing the elution, rinse the Aurum vacuum manifold with water, and air dry or wipe with paper towels. Cut tubing into 3 pieces of appropriate length. -
Page 8: The Vacuum Regulator
Manifold: Column The vacuum regulator allows complete control of the negative pressures inside Purification the Aurum vacuum manifold, allowing optimum levels of negative pressure to be achieved for efficient sample preparation. This is particularly important Protocol Overview when a gradual increase in negative pressure is required during 96-well plate For complete protocol, consult instruction manual. -
Page 9: Troubleshooting Guide
CAP to seal Vacuum Purification Mini Midi Maxi Unused CAP luer fittings Close unused luer not closed completely fittings completely with Aurum Vacuum Manifold • • • • caps Promega Vac-Man • Gasket residue preventing Rinse manifold with Promega Vac-Man 96 •... -
Page 10: Ordering Information
Section 10 Ordering Information Catalog # Description Problem Possible Cause Possible Solution 732-6470 A A u u r r u u m m v v a a c c u u u u m m m m a a n n i i f f o o l l d d , includes column adaptor plate, Worn manifold gasket Replace gasket 4 replacement luer caps, A and B stages, waste collection...
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