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Chapter: 3
Overview
A typical Nano ITC experiment involves the following:
•
Preparing and degassing the solutions
•
Preparing the sample and reference cells
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Mounting the buret
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Running the baseline
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Performing an analysis
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Cleaning the calorimeter
Each step is briefly described here. Additional information is provided in the online help supplied with the
software program. It is assumed that you are familiar with standard laboratory procedures and techniques.
It is critical that the Nano ITC cells be cleaned immediately at the end of each experiment. The calorimeter
can be left idle for up to two days with water-filled cells at 25°C, when not performing experiments. When
the instrument is expected to remain idle longer than five days, clean and empty the cells.
The Nano ITC can be calibrated using standardized chemical reactions. If suitable reagents are not
available, then as an alternate option perform the calibration using electrical pulses.
Nano ITC Getting Started Guide
Use, Maintenance, & Diagnostics
Page 23
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