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the plastic barrel gently during insertion to help the needle tip self-center in the sample access tube.
Figure 17 Inserting the buret into the calorimeter.
2
Gently push the buret handle downward and rotate it slightly clockwise to secure the buret in place. If
the buret handle does not easily slip fully into place into the Low Volume ITC, an incompatible
syringe may be in place. Do not attempt to force the buret downward or damage may occur. Remove
the buret handle and check the syringe type.
Drop the buret down slowly to allow enough time for the syringe needle to come into thermal equilibration
before it enters the sample cell. The System tab in the ITCRun software can be used to monitor this
thermal settling process.
When the buret is installed properly, the graduations will be facing directly forward. Circular contact
boards at the buret/calorimeter interface provide electrical power to the buret and enable the functional
control necessary to perform a titration.
Starting the Experiment
Once the loaded buret assembly is installed, you can proceed as follows:
1
Turn on the stirrer at an appropriate speed. For instruments with gold cells, this is typically in the range
of 250–400 rpm. Hastelloy cell instruments typically use 150–200 rpm.
2
Allow the system to re-equilibrate until the calorimeter heat reading is stable.
3
Set up the parameters for the particular experiment of interest using the ITCRun program under the
Setup tab (see the online help for details).
4
Use the provided data entry fields for syringe and cell concentrations. There is a comment field that can
be used to identify the sample chemicals, temperature, stirring speed, etc. This information will be visible
when the data is loaded into NanoAnalyze software.
5
Wait until a stable baseline is evident. (ITCRun™ software includes an optional equilibration detection
function which automatically starts the experiment when the desired signal stability has been established.)
Then click Experiment > Start or click the Go icon at the top of the toolbar to start the experiment.
6
Enter a filename when prompted. The program indicator on the Setup tab will be red when the program
is active. You will be able to watch the progress of the experiment under the Monitor tab or the Data tab.
7
The titration data is automatically saved to disk at the conclusion of the experiment.
8
Evaluate the data using the NanoAnalyze software package. (See the online help for information.)
Page 32
Nano ITC Getting Started Guide
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