Fisher Scientific Isotemp Series User Manual
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Fisher Scientific Isotemp Series User Manual

Gravity and convection incubators
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  • Page 2 This document is not part of any sales contract between Thermo Fisher Scientific and a purchaser. This document shall in no way govern or modify any Terms and Conditions of Sale, which Terms and Conditions of Sale shall govern all conflicting information between the two documents.

  • Page 3: Table Of Contents

    Sensing and Control System ....................... 4-7 Data Communications & Alarm Interface ..................4-8 RS-232 Interface..........................4-8 AC Power Socket..........................4-9 Fuses............................... 4-9 Work Space Components ........................4-9 Inner Chamber ..........................4-9 Access Port ............................. 4-9 Shelf System ............................. 4-10 Fisher Scientific Gravity Convection Incubators...
  • Page 4 Service Intervals..........................10-2 Preparing Temperature Calibration ....................10-2 Comparison Measurement Procedure ....................10-3 Temperature Calibration Procedure ....................10-3 Replacing the Door Seal ........................10-4 Replacing the Power Cord ........................ 10-4 Returns for Repair ..........................10-5 Gravity Convection Incubators Fisher Scientific...
  • Page 5 Contents Chapter 11 Disposal...........................11-1 Overview of Materials Used ......................11-1 Chapter 12 Error Codes........................12-1 Chapter 13 Technical Data ........................13-1 Chapter 14 Spare Parts and Accessories ..................14-1 Chapter 15 Device Log ........................15-1 Fisher Scientific Gravity Convection Incubators...
  • Page 6 Contents Gravity Convection Incubators Fisher Scientific...
  • Page 7 Figure 5-7 Removing the left and right support profiles..................5-5 Figure 5-8 Removing the rear air baffle........................5-5 Figure 5-9 AC Power Supply Socket ........................5-7 Figure 7-1 Control Panel for Fisher Scientific Incubators ..................7-1 Figure 10-1 Door Seal Replacement ........................10-4 Fisher Scientific...
  • Page 8 List of Figures Gravity Convection Incubators Fisher Scientific...

  • Page 9: Chapter 1 Safety Notes

    Basic Operating Precautions These operating instructions describe Fisher Scientific incubators. Fisher Scientific incubators have been manufactured to the latest state of the art and have been tested thoroughly for flawless functioning prior to shipping. However, the incubator may present potential hazards, particularly if it is operated by inadequately trained personnel or if it is not used in accordance with the intended purpose.

  • Page 10: Operational Safety Rules

    Operational Safety Rules Operational Safety Rules The following rules must be heeded when working with Fisher Scientific incubators: • Observe the sample weight limits specified for your Fisher Scientific incubator as a whole and its shelving in particular; see “Technical Data”...

  • Page 11: Explanation Of Safety Information And Symbols

    Indicates a hazardous situation which, if not avoided, could result in death or serious injuries. Indicates a situation which, if not avoided, could result in damage to equipment or property. Is used for useful hints and information regarding the application. Fisher Scientific Gravity Convection Incubators...

  • Page 12: Additional Symbols For Safety Information

    Explanation of Safety Information and Symbols Additional Symbols for Safety Information Wear safety gloves! Wear safety goggles! Harmful liquids! Electric shock! Hot surfaces! Fire hazard! Explosion hazard! Suffocation hazard! Biological hazard! Contamination hazard! Danger of tipping! Gravity Convection Incubators Fisher Scientific...

  • Page 13: Symbols On The Incubator

    Safety Notes Explanation of Safety Information and Symbols Symbols on the Incubator Observe operating instructions Mark of conformity USA/Canada 120 Volts AC power socket Fisher Scientific Gravity Convection Incubators...

  • Page 14: Intended Purpose Of The Incubator

    Intended Purpose of the Incubator Intended Purpose of the Incubator Correct Use Fisher Scientific incubators are laboratory devices for preparing and cultivating cell and tissue cultures. The devices employ precision temperature control for simulating the specific physiological ambient conditions for these cultures.

  • Page 15: Chapter 2 Delivery Of The Incubator

    Delivery of the Incubator Packaging Fisher Scientific incubators are delivered in a rugged packaging box. All packaging materials can be separated and are reusable: Packaging materials Packaging carton: Recycled paper Foam elements: Styrofoam (CFC-free) Pallet: Chemically untreated wood Packaging film:...

  • Page 16: Scope Of Supply

    Scope of Supply Incubators Gravity Convection Forced Air Quantity of components supplied (pieces) Incubators Incubators Perforated shelves Support rail for shelf table-top incubators Shelf support Power cord Clip springs for table-top incubators Plug Anti-tilt anchor Operating manual Gravity Convection Incubators Fisher Scientific...

  • Page 17: Chapter 3 Installation

    • Place the incubator on a floor stand (option; to be ordered separately), never on the lab floor. This prevents the penetration of dust or dirt into the device. • Power line voltage variations must not exceed ±10 % of the nominal voltage. Fisher Scientific Gravity Convection Incubators...

  • Page 18: Intermediate Storage

    • If excessive temperatures tend to occur in the operating room, be sure to provide a thermal protection means that cuts out the power supply to mitigate the impact of overtemperature scenarios. Gravity Convection Incubators Fisher Scientific...
  • Page 19 * Depth of handle /display (66 mm/2.6 in) not included in overall depth specified; height of adjustable feet (36 mm/1.4 in) not included in overall height specified. Table 3-2 Required Clearances E (mm/inch) F (mm/inch) G (mm/inch) H (mm/inch) 80/3.1 50/2.0 200/7.9 300/11.8 Fisher Scientific Gravity Convection Incubators...
  • Page 20 Protection Equipment, such as safety shoes, when lifting the incubator. To avoid crushing your fingers or hands (particularly in a closing door) or damaging the incubator, do not use any other lift points than those indicated in the illustration above. Gravity Convection Incubators Fisher Scientific...

  • Page 21: Installing The Anti-Tilt Anchor

    Fix the anti-tilt anchor to a solid part of the building. Floor stand incubators Fisher Scientific floor stand incubators must always be attached to the wall using two (2) retaining brackets on the outer left and right side on the back of the unit.
  • Page 22 To avoid injury to persons and damage to equipment, do not attempt to move stacked devices as a unit! Separate and move each device one by one, then restack them. Gravity Convection Incubators Fisher Scientific...

  • Page 23: Chapter 4 Product Description

    This section describes Fisher Scientific microbiological incubators: • Fisher Scientific Gravity convection incubators: • Fisher Scientific Forced air incubators. This section describes the Fisher Scientific microbiological incubators for standard laboratory applications. Gravity Convection Incubator Overview Gravity convection incubators come equipped with the following features: •...
  • Page 24 Door latch and handle Door hinge, lower Levelling foot Nameplate Perforated shelf Support rail for perforated shelf Shelf support [10] Door hook catch [11] Door seal [12] Stacking pad [13] Glass door [14] Temperature sensor Gravity Convection Incubators Fisher Scientific...
  • Page 25 Product Description Gravity Convection Incubator Overview Figure 4-2 Isotemp 60L/100L/180L Incbtr Grvity Rear View [3] Door bearing [4] Access port [5] Electronics compartment [6] Leveling foot Fisher Scientific Gravity Convection Incubators...

  • Page 26: Forced Air Incubator Overview

    75 °C (167 °F) • one speed work space fan At high ambient temperatures the maximum speed is reduced. • an on/off timer • two perforated shelves • an access port for tubing, sensor leads, etc. Gravity Convection Incubators Fisher Scientific...
  • Page 27 Support rail for perforated shelf Shelf support [10] Fan opening, air baffle [11] Door hook catch [12] Air baffle [13] Door seal [14] Stacking pad [15] Spring [16] Power outlet [17] Access port [18] - [19] Glass door Fisher Scientific Gravity Convection Incubators...
  • Page 28 Product Description Forced Air Incubator Overview Figure 4-4 Isotemp 60L/100L/180L Incbtr FA Rear View [3] Door bearing [4] Access port [5] Electronics compartment [6] Leveling foot Gravity Convection Incubators Fisher Scientific...

  • Page 29: Safety Devices

    The PT 100-type sensor for the control of the work space temperature and for the thermal protection [1] is installed on the bottom of table-top incubators and in the top of floor stand incubators. Figure 4-5 Sensor System (for table-top incubators) Figure 4-6 Sensor System (for floor stand incubators) Fisher Scientific Gravity Convection Incubators...

  • Page 30: Data Communications & Alarm Interface

    The RS- 232 interface (item 2 in figure 4-7 below) may be used to connect Fisher Scientific incubators to the serial interface port of a computer to allow for the computer-aided acquisition and documentation of major operating parameters (temperature, error codes, etc.).

  • Page 31: Ac Power Socket

    The device fuses are not user-serviceable. When the incubator exhibits the typical signs of a blown fuse (no response to pressing the On/Off button, control panel remains extinguished, no heating operation), call Fisher Scientific Customer Service to have the fuses replaced. Work Space Components Inner Chamber All components of the work space are made of corrosion-resistant stainless steel and have an absolutely smooth and easy-to-clean surface.

  • Page 32: Shelf System

    The shelves [2] have an integrated tilt protection and pull-out stop. For details on using the shelf system, see “Installing the Shelf System” page 5-1. Retaining Springs Support rails Shelf support Perforated shelves Figure 4-8 Gravity Convection Shelf System 4-10 Gravity Convection Incubators Fisher Scientific...
  • Page 33 Product Description Work Space Components Air Baffles Retaining Springs (only for table-top incubators) Support Rails Shelf Support Shelves Figure 4-9 Forced Air Shelf System Fisher Scientific Gravity Convection Incubators 4-11...
  • Page 34 Product Description Work Space Components 4-12 Gravity Convection Incubators Fisher Scientific...

  • Page 35: Chapter 5 Start-Up

    2. Push the retaining spring [1] into the guide on the support rail [2], making sure that the locking nub [3] on the retaining spring safely engages with the matching hole in the support rail. Figure 5-1 Sliding the Retaining Spring into the Support Rail Fisher Scientific Gravity Convection Incubators...

  • Page 36: Installing The Perforated Shelves

    Support rail (for floor stand units, air baffle) Shelf support, Perforated shelf Figure 5-2 Installing the Shelving Air Baffles Retaining Springs (only for table-top incubators) Support Rails Shelf Support Shelves Figure 5-3 Forced Air Shelf System Gravity Convection Incubators Fisher Scientific...

  • Page 37: Preparing The Work Space

    Preparing the Work Space Preparing the Work Space Upon delivery, Fisher Scientific incubators are not in a sterile state. Before the initial start-up, the incubator must be decontaminated. The following work space components should be checked for cleanliness and disinfected prior to use: •...

  • Page 38: Installing The Shelf Support Brackets

    2. Make sure that the two vertical elements [2] of the shelf support butt against the support rail. Figure 5-5 Shelf Support Installation Installing the Perforated Shelves Rear Pull-out Stop Anti-tilt anchor Front Pull-out Stop Shelf Figure 5-6 Installing the Perforated Shelves Gravity Convection Incubators Fisher Scientific...

  • Page 39: Installing/Removing Air Baffles

    1. Loosen and remove the four (4) screws in the bottom plate and then remove the bottom plate completely. Figure 5-8 Removing the left and right support profiles Loosen and remove the eight (8) screws for the left and right support profiles and then take out the lateral air baffles. Fisher Scientific Gravity Convection Incubators...

  • Page 40: Levelling The Table-Top Incubator Unit

    AC power cord supplied to connect the incubator to a correctly installed and protection-earthed power supply source, with the following features in place for each incubator: • T 16 A slow-blow fusing • B 16 circuit breaker • FI circuit breaker Gravity Convection Incubators Fisher Scientific...

  • Page 41: Connecting To The Power Supply Source

    Figure 5-10 AC Power Supply Socket Note The alarm contact is not functional with gravity convection incubators. If you have a need for alarming, please contact Fisher Scientific Customer Support for advice. Condensation When taking the incubator into operation for the first time allow some time before switching on for stabilization to avoid condensation forming on live parts.

  • Page 42: Connecting The Rs-232 Interface

    4. Connect the second connector to an unused COM 1 /COM 2 or other serial port on the computer. 5. Boot the computer. 6. Launch your standard terminal program and set up the connection with the following parameters: — 57600 bits per second — 8 data bits Gravity Convection Incubators Fisher Scientific...
  • Page 43 — <CR> is for carriage return. Table 5-1 Terminal Commands for Querying Data Command Syntax Response Example Combined Date and Time ?:0010:00::c1 !:0010:11:31.07.10;01:02:23:e2 Date Time Date only ?:0011:00::c0 !:0011:08:31.07.10:d2 Date Time only ?:0012:00::c3 !:0012:08:01:02:23:dc Time Fisher Scientific Gravity Convection Incubators...
  • Page 44 Start-up Connecting the RS-232 Interface Table 5-1 Terminal Commands for Querying Data Command Syntax Response Example Temperature Set Value (T1); Current Work Space Temperature (T2); Reference Temperature (T3); Sample Sensor Temperature (T4) ?:3010:00::c2 !:3010:1f:+125.00;+124.96;+000.000;+000.00:b0 5-10 Gravity Convection Incubators Fisher Scientific...

  • Page 45: Chapter 6 Operation

    “Incorrect Use” page 1-5 • make sure that the ambient air is free of any solvents • do not operate the incubator in areas with an explosion hazard 4. Load the work space with samples. Fisher Scientific Gravity Convection Incubators...
  • Page 46 To ensure sufficient air circulation and uniform heating of the samples, do not use more than 70% of the maximum surface area of the work space. Bulky objects in the work space that dissipate heat may impair heat distribution. Gravity Convection Incubators Fisher Scientific...

  • Page 47: Chapter 7 Handling And Control

    Handling and Control Fisher Scientific incubators come with a front panel mounted control unit consisting of a multifunctional display, four control buttons, and an on/off button. The four control buttons interact with the display window to let users access all of the user control functions and adjustments of the incubator, including - for example, the temperature set value, timer, energizing/de-energizing, as well as a variety of other functions.
  • Page 48 Escape button Returns to the previous level of the menu or standard display. Upon exiting from the current menu item the user may be prompted to save any previously made settings. Gravity Convection Incubators Fisher Scientific...
  • Page 49 Invokes a submenu with the following functions: - Read access to error log - Calibrating the oven - Toggling the temperature display unit between °C and °F - Entering a configuration control code (Instructions: “Settings” page 7-10) Fisher Scientific Gravity Convection Incubators...
  • Page 50 “Switching the Oven Off / Powering Down” page 7-5) Button Inactivity Monitoring When no button is pressed for more than 30 seconds in any selected menu item, the display exits from the selected screen and returns to default display mode. Gravity Convection Incubators Fisher Scientific...

  • Page 51: Powering Up

    7-1) and a residual heat temperature readout in case the work space temperature is still higher than 50 °C/122 °F. The oven is switched off now. 2. If required, unplug the AC power plug to power down the oven completely. Fisher Scientific Gravity Convection Incubators...

  • Page 52: Temperature Set Value

    Switching the Oven Off / Powering Down Temperature Set Value Fisher Scientific incubators allow for setting the desired work space temperature directly using only a few button presses. After confirming the new temperature set value, you may trace the resulting temperature change in the temperature display field (item D1 in...

  • Page 53: Timer

    Set the hours and minutes until the oven is supposed to shut down by pressing , then press confirm. The display returns to its default mode. In the menu bar, the Timer icon is illuminated and a hand is rotating on the icon's face. Fisher Scientific Gravity Convection Incubators...

  • Page 54: Stopping A Timer

    Additionally, the readiness indicator icon is illuminated. Stopping a Timer Table 7-7 Stopping an Off Timer Before It Expires Press to activate the menu bar, then use to select the Timer icon and press to confirm. Gravity Convection Incubators Fisher Scientific...
  • Page 55 Table 7-8 Stopping an On Timer To cancel a pre-programmed on timer while the incubator is switched off, hold the On/Off button depressed for a few seconds. In the menu bar, the Timer icon will go out. Fisher Scientific Gravity Convection Incubators...

  • Page 56: Settings

    Settings The Settings menu item opens a submenu populated with various commands for viewing general status information on the Fisher Scientific unit and setting for the operation of the incubator or its display window: • Read access to error log •...

  • Page 57: Calibration

    Table 7-10 Entering the Calibration Reference Temperature Manually Prepare for temperature calibration (see “Preparing Tempera- ture Calibration” “Comparison Measurement Procedure” page 10-3). Press to activate the menu bar, then use select the Settings icon and press to confirm. Fisher Scientific Gravity Convection Incubators 7-11...

  • Page 58: Temperature Display Unit

    Settings icon and press to confirm. Press to switch to the C - F menu item. The text C - F is flashing in the display pane. 7-12 Gravity Convection Incubators Fisher Scientific...
  • Page 59 The temperature unit to the right of the temperature display field (item D1 in figure 7-1 page 7-1) has changed according to your selection. The display returns to its default mode. Settings icon in the menu bar will go out. Fisher Scientific Gravity Convection Incubators 7-13...
  • Page 60 Handling and Control Switching the Oven Off / Powering Down 7-14 Gravity Convection Incubators Fisher Scientific...

  • Page 61: Chapter 8 Shut-Down

    4. Unplug the power cord and secure it against accidental reconnection. 5. Until the incubator is shut down, the work space must be continuously ventilated. Leave the glass door and the outer door open and secure them against accidental closure. Fisher Scientific Gravity Convection Incubators...
  • Page 62 Shut-down Shutting the Incubator Down Gravity Convection Incubators Fisher Scientific...

  • Page 63: Chapter 9 Cleaning And Disinfection

    Wipe the surfaces clean using a clean cloth and clear water. Then, wipe the surfaces dry using a clean cloth. Wipe / Spray Disinfection The manual wipe and spray disinfection is a three-stage process: • predisinfection, • cleaning, • final disinfection. Fisher Scientific Gravity Convection Incubators...

  • Page 64: Preparing The Manual Wipe/Spray Disinfection

    • Wear safety gloves. • Wear safety goggles. • Wear mouth and respiratory system protection gear to protect your mucous membranes. • Observe the safety instructions of the disinfectant’s manufacturer and the hygiene supervisor. Gravity Convection Incubators Fisher Scientific...

  • Page 65: Predisinfection

    Final Disinfection 1. Spray the specimen chamber surfaces and the internals removed from this chamber again with disinfectant, or wipe them down. 2. Let the disinfectant work on the surfaces/internals as detailed in the manufacturer’s instructions. Fisher Scientific Gravity Convection Incubators...
  • Page 66 Under specific circumstances, there is a risk that bacteria, viruses, fungi, prions, and other biological substances survive. Decontamination must be performed immediately if any biohazardous material is spilled in or on the incubator. Gravity Convection Incubators Fisher Scientific...

  • Page 67: Chapter 10 Maintenance

    • Perform electrical safety check in accordance with the relevant national regulations Functional Check If safety devices were removed or disabled for inspections, the incubator must not be operated before the safety devices have been reinstalled and checked for their correct function. Fisher Scientific Gravity Convection Incubators 10-1...

  • Page 68: Service Intervals

    3-monthly service • Perform the comparative temperature measurement outlined in the following section. Annual Service • Have the incubator inspected and services by a Fisher Scientific authorized Technical Service agent. Service contract Fisher Scientific International, LLC offer a product-specific service contract comprising all necessary tests and service work.

  • Page 69: Comparison Measurement Procedure

    For detailed instructions on how to perform a manual temperature calibration, please refer to the instructions in the section “Calibration” page 7-12. Excessive work space temperature Excessive work space temperatures after the calibration can be reduced by leaving the doors open for approx. 30 seconds. Fisher Scientific Gravity Convection Incubators 10-3...

  • Page 70: Replacing The Door Seal

    Correct, if necessary. Replacing the Power Cord If the power cord is damaged, it must be replaced by an original spare part. Using a standard power cord with a lower thermal withstand capabilities is prohibited. 10-4 Gravity Convection Incubators Fisher Scientific...

  • Page 71: Returns For Repair

    • Clean the incubator components thoroughly, then disinfect or decontaminate them (depending on application). • Fill in and attach a safety declaration with details on decontamination activities performed to the items that are to be repaired. Fisher Scientific Gravity Convection Incubators 10-5...
  • Page 72 Maintenance Returns for Repair 10-6 Gravity Convection Incubators Fisher Scientific...

  • Page 73: Chapter 11 Disposal

    Device rear panel Galvanized steel sheet Outer door Galvanized steel sheet, painted, + stain- less steel (optional) Door inner panel Stainless steel Control panel and display window Polyethylene protective foil Heater Silicone-sheathed resistance heater wires Fisher Scientific Gravity Convection Incubators 11-1...
  • Page 74 Interior containers, installed compo- Stainless steel 1.4016 + 1.4301 nents and shelves Door frame seal Silicone Glass screen Sodium silicate glass Cables Plastic-sheathed stranded copper wire Packaging Corrugated board, polyethylene film, and styrofoam, chemically untreated wood 11-2 Gravity Convection Incubators Fisher Scientific...

  • Page 75: Chapter 12 Error Codes

    (see “Error Log” page 7-11) and provides instructions for clearing such alarms. Table 12-1 Fisher Scientific Incubator Error Codes Error Message & Code Root Cause Alarm Response Alarm Clearing Instructions...
  • Page 76 Error Codes Table 12-1 Fisher Scientific Incubator Error Codes Error Message & Code Root Cause Alarm Response Alarm Clearing Instructions Incorrect voltage The applied voltage is too Audible alarm activated, Apply the correct voltage (E014) high or too low. error message shown on as indicated on the name- display.
  • Page 77 Error Codes Table 12-1 Fisher Scientific Incubator Error Codes Error Message & Code Root Cause Alarm Response Alarm Clearing Instructions Heating Triac Error The voltage measurement Audible alarm activated, Call service and switch the (E110) has indicated a defect in message shown on dis- device off.
  • Page 78 Error Codes 12-4 Gravity Convection Incubators Fisher Scientific...

  • Page 79: Chapter 13 Technical Data

    738/ 25.2 25.2 29.1 Overall weight kg/lbs 40/88 51/112 65/143 Loading capacity Loading capacity per shelf kg/lbs 25/55 Max. overall loading capacity per device kg/lbs 50/110 50/110 75/165 Electrical data Power consumption Maximum current Fisher Scientific Gravity Convection Incubators 13-1...
  • Page 80 Minimum side clearance mm/in 50/2 Minimum front clearance mm/in 590 / 23.2 690 / 27.2 814 / 32 Minimum back wall clearance mm/in 80/3.2 Minimum bottom clearance mm/in 200/8 Minimum top clearance mm/in 300/12 13-2 Gravity Convection Incubators Fisher Scientific...
  • Page 81 Earthing system (e. g. 1/N/PE) 1/N/PE 1/N/PE 1/N/PE Power line frequency Power line voltage +/- 10 % IP protection system IP 20 Protection class Overvoltage category Device fusing, building side Device fusing, on PCB 2 x 16 Fisher Scientific Gravity Convection Incubators 13-3...
  • Page 82 Minimum side clearance mm/in 50/2 Minimum front clearance mm/in 590 / 23.2 690 / 27.2 814 / 32 Minimum back wall clearance mm/in 80/3.2 Minimum bottom clearance mm/in 200/8 Minimum top clearance mm/in 300/12 13-4 Gravity Convection Incubators Fisher Scientific...

  • Page 83: Chapter 14 Spare Parts And Accessories

    Kit door seal for gravity convection incubator, 180L with ventilation cut-out 50127436 Kit door seal for forced air incubator, 60L 50127437 Kit door seal for forced air incubator, 100L 50127438 Kit door seal for forced air incubator, 180L Fisher Scientific Gravity Convection Incubators 14-1...
  • Page 84 Spare Parts and Accessories 14-2 Gravity Convection Incubators Fisher Scientific...

  • Page 85: Chapter 15 Device Log

    Device Log Incubator type: Part number: Serial number: Service number: Location Operator’s note: Work carried out Notes Date Signature Fisher Scientific Gravity Convection Incubators 15-1...
  • Page 86 Device Log 15-2 Gravity Convection Incubators Fisher Scientific...
  • Page 87 © 2014 Thermo Fisher Scientific Inc. All rights reserved. Trademarks used are owned as indicated at www.fishersci.com/trademarks. In the United States: For customer service, call 1-800-766-7000 To fax an order, use 1-800-926-1166 To order online: www.fishersci.com...

This manual is also suitable for:

Isotemp 60l incbtr grvity 120vIsotemp 100l incbtr grvity 120vIsotemp 180l incbtr grvity 120vIsotemp 100l incbtr fa 120vIsotemp 180l incbtr fa 120vIsotemp 60l incbtr fa 120v

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