Predefined Phases - GE AKTAavant Getting Started

6.1.3 Predefined phases

The predefined phases that are available in the predefined methods and in the Phase
Library are described in the table below.
Phase
Method Settings
Equilibration
Sample Application
Column Wash
Elution
Column
Preparation
Column CIP
System
Preparation
System CIP
Column
Performance Test
Getting Started with ÄKTAavant and UNICORN 6 28-9440-69 AB
Description
The first, and mandatory, phase in any method. Defines
common parameters used in the subsequent phases.
Equilibrates the column before purification, or re-equilibrates
the column after purification.
Applies sample to the column. Defines the sample application
technique, the sample volume, and the handling of
flowthrough.
Washes out unbound sample after sample application or
removes strongly bound proteins after elution.
Elutes the sample from the column. Defines parameters for
the elution and fractionation settings.
Prepares the column before use by removing the storage
solution and equilibrating the column. By adding steps, several
preparation solutions can be used sequentially.
Cleans the column after purification runs by rinsing the column
with a cleaning solution to remove unspecifically bound
proteins. By adding steps, several cleaning solutions can be
used sequentially.
Prepares the system before a run by removing storage solution
and filling the system and inlets with buffer solution. One
preparation solution is used per phase.
Cleans the system after purification runs by rinsing the system
with a cleaning solution. One cleaning solution is used per
phase.
Tests the efficiency of a packed column in terms of height
equivalent to a theoretical plate (HETP), and the peak
asymmetry factor (A ).
s
6 Create a method
6.1 Guide to method creation
6.1.3 Predefined phases
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