NEW BRUNSWICK SCIENTIFIC AF 2000 User Manual
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NEW BRUNSWICK SCIENTIFIC AF 2000 User Manual

Anti–foam control module
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UNITED KINGDOM
New Brunswick Scientific (UK) Limited
Edison House–163 Dixons Hill Road
North Mymms Hatfield Hertfordshire
AL9 7JE United Kingdom
Tel: 07072 75733
Fax: 07072 67859
Telex: 94018208 NBSC G
AF 2000
Anti–Foam Control Module
MANUAL NO: M1230–0053
Rev E
March 4, 1998
NEW BRUNSWICK SCIENTIFIC CO., INC
BOX 4005 . 44 TALMADGE ROAD . EDISON, NJ 08818-4005
TELEPHONE: . 732-287-1200 . 800-631-5417 . FAX: 732-287-4222
TELEX: 4753012 NBSCO . INTERNET: http://www.nbsc.com
E-MAIL: bioinfo@nbsc.com
THE NETHERLANDS
New Brunswick Scientific Benelux B.V.
P.O Box 6826
6503 GH Nijmegen
Holland
Tel: 080 3779179
Fax: 080 3787755
BELGIUM
New Brunswick Scientific NV / SA
't Veldeke, 1 B-1970
Wezembeek-OPPEM
België/Belgique
Tel: 02 731 67 87
Fax: 02 731 81 30
.
GERMANY
New Brunswick Scientific GMBH
In Der Au 14
D-72622 Nürtingen
Deutschland
Tel: 07022 932490
Fax: 07022 32486

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  • Page 1 THE NETHERLANDS BELGIUM GERMANY New Brunswick Scientific (UK) Limited New Brunswick Scientific Benelux B.V. New Brunswick Scientific NV / SA New Brunswick Scientific GMBH Edison House–163 Dixons Hill Road P.O Box 6826 ‘t Veldeke, 1 B-1970 In Der Au 14...
  • Page 2 CAUTION ! CAUTION ! Operate the AF 2000 as described in this manual. Operate the AF 2000 as described in this manual. If Operational Guidelines are not followed, equipment damage can If Operational Guidelines are not followed, equipment damage can occur.
  • Page 3 Reproduction, adaptation or translation without prior written permission from New Brunswick Scientific is prohibited. Disclaimer Notice New Brunswick Scientific Company Inc., reserves the right to change information in this document without notice. Updates to this information reflect our commitment to continuing product development and improvement.
  • Page 4 W A R R A N T Y Every Instrument manufactured by the New Brunswick Scientific Co., Inc. is warranted to be free from defects in material and work- manship. This apparatus with the exception of glassware, lamps and electrodes (where supplied), is warranted for 1 year against faulty com-...

  • Page 5: Table Of Contents

    ODULES 6.2 A –F ........18 ODULE NTERCONNECTING ABLE ONNECTION 7. PREPARATION BEFORE THE AUTOCLAVE ............19 7.1 V ................19 ESSEL LATE SSEMBLY 7.2 P ........23 REPARING THE ONDUCTANCE ROBE FOR THE UTOCLAVE New Brunswick Scientific Co., Inc.
  • Page 6 11. REPLACEMENT PARTS AND ACCESSORY INFORMATION ......31 11.1 R ........31 EPLACEMENT ARTS ESCRIPTIONS AND PART UMBERS 11.2 A ....31 CCESSORY ESCRIPTIONS AND ONDUCTANCE ROBES UMBERS APPENDIX A Control Schematic–AF 2000..............32 INDEX ........................... 33 AF 2000 User’s Guide...
  • Page 7 This page intentionally blank. New Brunswick Scientific Co., Inc.

  • Page 8: Overview

    OVERVIEW The New Brunswick Scientific's AF 2000 Anti–Foam Module is an optional unit that connects to the BioFlo 2000 Fermentor. The Anti–Foam Module regulates the operation of a self contained feed pump that delivers anti–foam agent and limits the agitation rate when high foam condition occur.

  • Page 9: Specifications

    NSPECTION OF OXES After you have received your order from New Brunswick Scientific, inspect the boxes carefully for any damage that may have occurred during shipping. Report any damage to the carrier and to your local NBS Sales Order Department.

  • Page 10: Preparing The Location

    Modules. NVIRONMENT The Anti–Foam Module operates properly under the following conditions: • ambient temperature range 10°C–35°C • relative humidity up to 80% non-condensing • voltage fluctuation ±10% AF 2000 User’s Guide...

  • Page 11: Unpacking & Inspection

    Customer Satisfaction Form 6300 and return by fax or mail. You can also call the New Brunswick Scientific Service Department at (732) 287-1200 / 800-237-2298. To properly unpack the AF 2000 perform the following steps: 4.1.1 Open the top of the box.
  • Page 12 SPARE FUSE GROUND POST SCREW Anti–Foam Module User’s Guide ANTI–FOAM MODULE USER’S GUIDE ANTI–FOAM MODULE INTERCONNECTING CABLE 15 PIN 4 SUPPORT PILLARS CONDUCTANCE PROBE HEADPLATE FITTING AF 2000 User’s Guide...

  • Page 13: Anti-Foam Module Features

    • Located on front left side of the FOAM PROBE INPUT Anti–Foam Module–Connector CONDUCTANCE PROBE Lead • Located on the left side of the HIGH FOAM PROBE INPUT front panel– Connector for CONDUCTANCE PROBE LEAD New Brunswick Scientific Co., Inc.
  • Page 14 Foam Pump Head–Selects Pump operating modes • Located on the right front panel– ANTI–FOAM PUMP Adds Anti–Foam agent to the fermentation while the is in CONDUCTANCE PROBE contact with foam–Addition rate is controlled by the Bio Flo 2000 Fermentor AF 2000 User’s Guide...
  • Page 15 FOAM SENS PROBE AUTO GRND HIGH SENS PROBE PRIME NEW BRUNSWICK SCIENTIFIC Edison NJ U.S.A. Ground (GRND) Connector High Foam Probe Input Anti–Foam Pump On / Off Rocker Power Switch High Foam Sensitivity (SENS) Adjustment New Brunswick Scientific Co., Inc.

  • Page 16: Getting Started

    Anti–Foam Module Back Panel AF 2000 FUSE 2 AMP 15 Pin Power / Control Connector Fuse Receptacle GETTING STARTED AF 2000 User’s Guide...

  • Page 17: Stacking Control Modules

    Verify that the rubber grommets on the bottom of the additional Control Module match up with the on the top of the SUPPORT PILLARS Anti–Foam Module. 6.1.5 Push the additional Control Module down, and verify that the Module sits securely on the Anti–Foam Module. New Brunswick Scientific Co., Inc.

  • Page 18: Anti -Foam Module Interconnecting Cable Connection

    INTERCONNECTING CABLE AF 2000 CONNECTOR back panel of the BioFlo 2000 Fermentor. BioFlo 2000 Fermentor Back Panel AF 2000 Connector RS 422/232 Recorder pH 2000 FP 2000 AF 2000 XX 2000 Cooling Heater Valve AF 2000 User’s Guide...

  • Page 19: Preparation Before The Autoclave

    Detail Knurl Nut Back Ferrule Front Ferrule 7.1.3 Verify that the are properly inserted in FRONT BACK FERRULES CONDUCTANCE PROBE FITTING 7.1.4 Replace one of the screws with a VESSEL MOUNTING ARM GROUND POST SCREW New Brunswick Scientific Co., Inc.
  • Page 20 Thermowell Inoculation Sample/Harvest Front NOTE: When you use a and a simultaneously, use the FOAM PROBE LEVEL PROBE for the Probes. Use the FOAM/LEVEL PORT SPARE/EXHAUST PORT (M1230–9413) on the EXHAUST PORT CONDENSER SPARE/EXHAUST CONDENSER PORT AF 2000 User’s Guide...
  • Page 21 5 Liter Vessel HeadPlate Cooling Coil 1 Cooling Coil 2 Sample/Harvest Spare/Exhaust Spare/Exhaust Condenser Foam Ground Post Thermowell pH Electrode Sparger DO Electrode 3 Way Inlet Inoculation Spare Spare/Level Front New Brunswick Scientific Co., Inc.
  • Page 22 10 Liter Vessel HeadPlate Spare Cooling Coil 1 Cooling Coil 2 Spare/Exhaust Exhaust Foam Sparger Ground Post pH Electrode Thermowell Sample/Harvest DO Electrode 3 Way Inlet Spare/Level Spare Inoculation Spare Front AF 2000 User’s Guide...

  • Page 23: Preparing The Conductance Probe For The Autoclave

    RETAINING NUT will damage the Teflon coating on the probe and cause a short circuit. NOTE: The Sterility of the will be compromised if the VESSEL CONDUCTANCE PROBE is readjusted after the is autoclaved. VESSEL New Brunswick Scientific Co., Inc.

  • Page 24: Fermentation Setup

    GROUND LEAD GRND CONNECTOR front panel of the Anti–Foam Module. NOTE: If you are using two with the AF 2000, only one CONDUCTANCE PROBES is connected to the Module. GROUND LEAD 8.1.4 Connect the other end of the...

  • Page 25: Anti -Foam Control

    . When foam contacts the sensing probe connected to the INPUT FOAM PROBE , the AF 2000 begins to add increments of anti–foam agent until the foam INPUT subsides. The duty cycle of the is fixed to allow time for the ANTI–FOAM PUMP...
  • Page 26 CONDUCTANCE PROBE HIGH on the front panel of the AF 2000. When foam contacts the FOAM PROBE INPUT high foam sensing probe, the AF 2000 adds anti–foam agent continuously until the foam subsides.

  • Page 27: Displaying The Current Values

    FOAM These values can be interpreted from the following table: Sensor Status Table Foam Value Foam Sensor High Foam Sensor No Foam No Foam Foam No Foam No Foam No Foam Foam Foam PREVENTIVE MAINTENANCE New Brunswick Scientific Co., Inc.

  • Page 28: Cleaning Procedures

    ON / OFF ROCKER POWER SWITCH 9.2.2 Disconnect the from ANTI–FOAM MODULE INTERCONNECTING CABLE the AF 2000 to the BioFlo 2000 Fermentor. 9.2.3 Use a small flat head screwdriver and place the tip of the screwdriver into the slot on the FUSE SOCKET 9.2.4...
  • Page 29 Use the screwdriver and gently press in and turn the FUSE SOCKET clockwise. 9.2.10 Reconnect the to the ANTI–FOAM MODULE INTERCONNECTING CABLE BioFlo 2000 Fermentor. Fuse Replacement AF 2000 FUSE Fuse Notches 2 AMP Fuse Socket New Brunswick Scientific Co., Inc.

  • Page 30: Troubleshooting

    SENS Adjustment is set Turn adjustment clockwise to too low increase sensitivity Conductance Probe connection Reconnect Conductance Probe Lead is open between AF 2000 and Conductance Probe GRND connection to Vessel is Reconnect GRND LEAD between open AF 2000 and HEADPLATE GROUND POST...

  • Page 31: Replacement Parts And Accessory Information

    REPLACEMENT PARTS AND ACCESSORY INFORMATION When ordering replacement parts, accessory parts or requesting service information, please provide the Model Number, Manufacturing Part Number and Serial Number of your AF 2000. This information is on the on the left ELECTRICAL SPECIFICATION PLATE side panel of the unit.

  • Page 32: Appendix A Control Schematic-Af 2000

    ON/OFF SWITCH 15 WAY D 15 WAY D ANTI FOAM 10 VAC HI FOAM FOAM/LEVEL TO SW2 FOAM MOD 18 VAC M1230-7001 GRN/YEL TO SW2 FRONT PANEL FRONT PANEL PUMP SSR1 PRIME ANTI FOAM CONTROL AUTO AF 2000 User’s Guide...

  • Page 33: Index

    · 25 sensitivity adjustment · 24 Foam Probe Input · 13 Foam Sensitivity (SENS) Adjustment · 14 Fuse Receptacle–2 Amp · 15 Fuse Replacement · 28 Schematic Getting Started af 2000 · 32 New Brunswick Scientific Co., Inc.
  • Page 34 Specifications · 9 Support Pillars · 13 Vessel HeadPlate 10 liter–graphic · 22 Vessel HeadPlate 2 liter–graphic · 20 Vessel HeadPlate 5 liter–graphic · 21 Vessel HeadPlate Assembly · 19 Troubleshooting · 30 troubleshooting table · 30 AF 2000 User’s Guide...

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