Viewing Window; Vessel Jacket - NEW BRUNSWICK SCIENTIFIC BioFlo 4500 Manual To Operations

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2.1.1

Viewing Window

The vessel includes a rectangular viewing window (approx. 1¼" W X 8½"
H, 3.18 cm W X 21.6 cm H) for observation of the vessel interior. There is
also a removable light port with a high-intensity lamp to facilitate viewing.
2.1.2

Vessel Jacket

• The vessel jacket houses ports for water circulation (not shown), and a
pressure relief valve.
• There are four cartridge heaters (675W in the 20L vessel/850W in the
30L vessel) at the bottom of the jacket (not shown). These are used to
heat the vessel to the required growth temperature. During initial
steam sterilization of the vessel, these heaters are not used.
LAMP with
PUSHBUTTON
COMBINED FILLING
(powder/liquid) /
LIGHT PORT
BAFFLES
(4, removable)
SIX-BLADED RUSHTON
IMPELLERS (2)
HEMISPHERICAL
DISH BOTTOM
HARVEST/DRAIN/
SAMPLE PORT
BioFlo 4500 M1254-0050
Figure 2: Vessel Cross Section
MOTOR
RUPTURE DISK
RUPTURE DISK
PIPING
IMPELLER SHAFT
SET SCREW
BAFFLE
RING SPARGER
User's Guide