Outlet Buffer Tray; Running The Assay; Launching The Sds Mw Instrument - Beckman Coulter PA 800 plus Application Manual

SDS-MW Analysis Kit

Running the Assay

Outlet Buffer Tray

Table 1.4 Outlet Buffer Tray Configuration
H2O
(Cycle 17-24)
H2O
(Cycle 9-16)
H2O
(Cycle 1-8)
H2O
(Cycle 17-24)
H2O
(Cycle 9-16)
H2O
(Cycle 1-8)
A
A1 to A6 – DDI H2O, use for dip step to clean capillary tip, 1.5 mL.
B4 to B6 – DDI H2O, use for dip step to clean capillary tip, 1.5 mL.
B1 to B3 – Waste vial for SDS-MW Gel rinse, 0.8 mL of DDI water.
C1 to C3 – SDS-MW Gel Buffer for separation (Gel-S), 1.1 mL.
D1 to D3 – Waste vial for 0.1N NaOH rinse, 0.8 ml of DDI water.
E1 to E3 – Waste vial for 0.1N HCl rinse, 0.8 ml of DDI water.
F1 to F3 – Waste vial for DDI H2O rinse, 0.8 ml of DDI water.
Running the Assay

Launching the SDS MW Instrument

1
Launch 32 Karat software. The Enterprise screen will display the available instruments.
2
Double-click on the
1-12
H2O
(Cycle 17-24)
H2O
(Cycle 9-16)
H2O
(Cycle 1-8)
Waste Gel-S
(Cycle 17-24) (Cycle 17-24)
Waste Gel-S
(Cycle 9-16) (Cycle 9-16)
Waste Gel-S
(Cycle 1-8) (Cycle 1-8)
B C
instrument to launch the SDS-MW assay.
SDS MW
Waste Waste
(Cycle 17-24) (Cycle 17-24)
Waste Waste
(Cycle 9-16) (Cycle 9-16)
Waste Waste
(Cycle 1-8) (Cycle 1-8)
D E
Waste
(Cycle 17-24)
Waste
(Cycle 9-16)
Waste
(Cycle 1-8)
F
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