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Document Ref:
0901814c
Date:
Monday, October 15, 2018
This document was prepared by the staff of Valeport Limited, the Company, and is the property of the
Company, which also owns the copyright therein. All rights conferred by the law of the copyright and by virtue
of international copyright conventions are reserved to the Company. This document must not be copied,
reprinted or reproduced in any material form, either wholly or in part, and the contents of this document, and
any method or technique available therefrom, must not be disclosed to any other person whatsoever without
the prior written consent of the Company.
Valeport Limited
Tel:
St Peters Quay
e mail:
Totnes
Web:
Devon, TQ9 5EW
United Kingdom
As part of our policy of continuous development, we reserve the right to alter, without prior notice, all
specifications, designs, prices and conditions of supply for all our equipment.
Hyperion Fluorometer
Operating Manual
+44 1803 869292
sales@valeport.co.uk | support@valport.co.uk
www.valeport.co.uk
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Summary of Contents for Valeport Hyperion
- Page 1 Monday, October 15, 2018 This document was prepared by the staff of Valeport Limited, the Company, and is the property of the Company, which also owns the copyright therein. All rights conferred by the law of the copyright and by virtue of international copyright conventions are reserved to the Company.
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Page 2: Table Of Contents
Table of Contents Table of Contents 1. EU Declaration of Conformity - CE Marking ............................. 3 2. Introduction - Hyperion Fluorometer ............................. 5 2.1. Fluorophore & Nomenclature ............................ 5 3. Sensors ............................. 6 3.1. Fluorometers ............................ 6 3.1.1 Safety Statement ............................ -
Page 3: Eu Declaration Of Conformity - Ce Marking
EU Declaration of Conformity - CE Marking 1. EU Declaration of Conformity - CE Marking Page 3 © 2018 Valeport Ltd... - Page 4 Please note: Any changes or modifications to the product or accessories supplied, that are not authorised by Valeport Ltd, could void the CE compliance of the product and negate your authority to operate it. This product has demonstrated CE compliance under conditions that include the use of shielded cables.
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Page 5: Introduction - Hyperion Fluorometer
AUV integration or used as part of a multi-sensor array and data logger. Offered as standard in a 6000m depth rated, Titanium housing the Hyperion Fluorometer has a wide range (9-30V DC) isolated power supply, data output up to 32Hz and RS232 communications. -
Page 6: Sensors
3.1. Fluorometers 3.1.1. Safety Statement A Hyperion Fluorometer is classified as Risk Group 1 under standard 62471. As the type is classified as Risk Group 1 solely due to radiation in the visible band a hazard label is not required. However, ·... -
Page 7: Fluorescein
2 gain settings, 0-50, 0-1000 (software controlled) Instrument <0.01 ppb* Detection limit: Actual 0.06 ppb** Detection limit: Linearity: 0.99 R Response Time: 0.03 to 2 s * 3x SD in RO water ** Calibrated against Rhodamine solution Page 7 © 2018 Valeport Ltd... -
Page 8: Linear Observation Range
(dependent on fluorophore properties, optical filters, LED power, sample volume and optical path length). Hyperion Fluorometers have a calibrated linear response for 2 gain settings (e.g. the ranges 0-40 µg/l (G5) and 0-800 µg/l (G1) for chlorophyll a). At higher concentrations, unlike analogue devices which generally flat-line at full-scale deflection (e.g. -
Page 9: Quenching
Photo-bleaching is dependent on exposure (intensity of light and duration) and wavelength (UV is more damaging than longer wavelengths). Use of more robust fluorophores is recommended to avoid photo-bleaching. * Reverse Osmosis ** De-Ionised Page 9 © 2018 Valeport Ltd... -
Page 10: Turbidity
A photometric test report was commissioned by Valeport in accordance with BS EN 62471. For this the LED was set to 25 times the operational power and the sensor was classified as exempt. However, it is best practice to avoid extended exposure to the LED and it is recommended not to look directly into the sensor windows. -
Page 11: Data Acquisition
During the synchronised observation period ambient light is measured while the Hyperion LED is off and the fluorescence signal of the fluorophore when the LED is on in order to cancel out the ambient light effects. - Page 12 0901814c - Hyperion Fluorometer For dye tracing applications, we recommend using the Hyperion at the default range, that is, high gain\sensitivity to reduce the quantity of dye required and, therefore, the visibility / load on the environment and or disposal costs.
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Page 13: Output Rate
4.3. Functional Check To perform a functional test connect the Hyperion to both pow er and PC using the supplied Y cable. Run DataLog x2, available from the Valeport Website as a free download - www .valeport.co.uk/... - Page 14 0901814c - Hyperion Fluorometer Leave this dialogue in its default settings, then select Next: The x2 program will now interrogate the instrument and get the settings and display them, then select Next: © 2018 Valeport Ltd Page 14...
- Page 15 Data Acquisition Set your operational mode and Instrument Range setting, then select Next: If there are any new settings to be up loaded to the instrument pressing Finish will send them: Page 15 © 2018 Valeport Ltd...
- Page 16 0901814c - Hyperion Fluorometer Check that data is being received (field 4) see Data Output Format Now check the following: The LEDs are on and light is being emitted from the fibres. The magnitude of the data received increases when the supplied fluorescent target is held at 45° to...
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Page 17: Operation With Midas Ctd
Operation with MIDAS CTD+ 5. Operation with MIDAS CTD+ If the Hyperion Instrument is used with a MIDAS CTD+ it should be configured using DataLog x2 software as follows: Baud Rate: 38400 Mode: Continuous The MIDAS CTD+ must be configured using DataLog Pro ver 04007125F1 or later Page 17 ©... -
Page 18: Electrical
0901814c - Hyperion Fluorometer 6. Electrical Voltage: 9 - 30V DC isolated Power: 40mA @ 12V DC 6.1. Connector Pin-Out Function RS232 GND Tx 232 out from SVS (485A) Rx 232 in to IPS (485B) +V(8-20V) /Enable 485 Note: to enable RS485 comms, link pins 1 and 5. -
Page 19: Communications
Baud Rate 2400 - 115200 Protocol 8 data bits, 1 stop bit, No parity, No flow control 7.1. Data Output Formats The Hyperion outputs a single NMEA style data telegram Example: $PVHYP,01,01,1234.45, 1.2345, 01, C02,*7F Where: Field Number Description... -
Page 20: Operating Modes
Other operational modes and filter settings are available - please contact Valeport with your specific requirements. 7.2.1. Mode C - Continuous Mode C is the default setting for Hyperion. -
Page 21: Hash (#) Codes
Read format string #105 #126 parameter_id Read parameter id #128 parameter_units Read units #222 averaging_constant Set the number of samples to average #223 averaging_constant Read the averaging length #401 fluoro_gain Report Fluorometer gain Page 21 © 2018 Valeport Ltd... -
Page 22: Physical Characteristics
0901814c - Hyperion Fluorometer 8. Physical Characteristics Materials: Titanium with glass window Polymer optical fibre Depth Rating: 6000m Dimensions: 40mmØ x 179.5mm (including connector) Weight 0.50 kg (in air) 0.26 kg (in water) Operating Temperature: 60°C max (without damaging the instrument) 8.1. -
Page 23: Care And Maintenance
Note: Organic solvents should not be used to clean the Hyperion. Strong acids and bases should be avoided. If fouling of the window is considerable, the unit should be returned to Valeport for servicing. Page 23 © 2018 Valeport Ltd... -
Page 24: Software - Datalog X2
0901814c - Hyperion Fluorometer 10. Software - DataLog x2 The Hyperion Fluorometers are supplied with DataLog x2 windows software DataLog x2 Real Time Display Charting is only possible once data is being recorded © 2018 Valeport Ltd Page 24... -
Page 25: Ordering And Part Numbers
Ordering and Part Numbers 11. Ordering and Part Numbers Fluorometer Part No. DESCRIPTION 0901001-C Hyperion-C, Chlorophyll a Fluorometer. Titanium housed, 6000m rated, Supplied with: - 0.5m interface cable - DataLog x2 software - Manual and transit case 0901001-F Hyperion-F, Fluorescein Fluorometer.