Scanning With The Vwd - Agilent Technologies G1314B VWD User Manual

Scanning with the VWD

Access to the scan feature is only possible during run.
N O T E
1 Set up a run.
2 Start a run.
3 While running on the baseline, select from the menu
Figure 22
4 When the peak of interest enters the flow cell, stop the flow (set flow rate to
Turning off the pump would stop the run and no access to the sample scan is possible.
N O T E
1200 Series Variable Wavelength Detector User Manual
Instrument - More VWD - Blank Scan, see Figure 22 on page 57.
A background scan is stored in the memory.
Taking Online Spectra
zero or open the purge valve) and wait a few moments to stabilize the
concentration.
• Step 1: Blank Scan: scan of the background
(solvent) is stored in the memory.
• Step 2: Sample Scan: scan of the peak of
interest is taken while the peak stays in the
flow cell (stop-flow condition).
• Online Spectrum: Sample Scan minus
Blank Scan.
Using the Detector
57
4