Integra ASSIST 4500 Operating Instructions Manual
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Summary of Contents for Integra ASSIST 4500
- Page 1 ASSIST Operating instructions 127950_V09...
- Page 2 Declaration of conformity INTEGRA Biosciences AG – 7205 Zizers, Switzerland declares on its own responsibility that the device Description Model ASSIST 4500 complies with: EU Directives (DoW: Date of Withdrawal) Before DoW After DoW Low Voltage Equipment 2006/95/EC 20.04.2016 2014/35/EU...
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Page 3: Table Of Contents
3.6.7 Move Speed................13 Chapter 4 Operation 4.1 Turn on/off the ASSIST device ............14 4.2 Turn on/off the VIAFLO Electronic Pipette ........14 4.3 Bluetooth connection..............14 4.4 Running a program.................14 4.5 Pipetting in 96 and 384 well plates ..........15 4.6 Troubleshooting................16 www.integra-biosciences.com... - Page 4 7.3 Intellectual Property................28 7.4 Compatible pipettes................29 7.5 Maximum labware height on plate carrier ........29 7.6 Tip travel distances ................30 Chapter 8 Accessories 8.1 Accessories for ASSIST ..............31 8.2 Accessories for VIAFLO-Pipettens ..........31 8.3 Consumables .................32 Imprint ....................35 INTEGRA Biosciences AG...
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Page 5: Chapter 1 Introduction
This symbol identifies important notes regarding the correct operation of the device and labor-saving features. The device is marked with the following symbol: IOHAZARD The instrument can be potentially biohazardous due to the use of biozazardous substances by the operator. www.integra-biosciences.com... -
Page 6: Intended Use
This is a general-purpose laboratory instrument for use in research only. Any use of this instrument in a medical or IVD setting is the sole responsibility of the user. If the ASSIST is used in a manner not specified by INTEGRA Biosciences, the protection provided by the ASSIST may be impaired. -
Page 7: Safety Notes
Regardless of the listed safety notes, additional applicable regulations and guidelines of trade associations, health authorities, trade supervisory offices, etc. must be observed. Please visit our website www.integra-biosciences.com on a regular basis for up to date information regarding REACH classified chemicals contained in our products. -
Page 8: Description Of The Device
Green LED blinks: press to start operation/homing Green LED lights: operation performed 14 Bluetooth control LED (lights blue if connection is active) and Error LED (flashes red in case of error) 15 On/Off key (lights green if On) INTEGRA Biosciences AG... -
Page 9: Overview Of Viaflo-Pipetten
24 Finger hook, facilitates easy operation 25 Volume indicator label, color matches GripTip box insert. 2.3.2 Display The Display shows all pipetting options. Battery life indicator OK - Select a program category and a program Current category Instruction Active step Volume Options www.integra-biosciences.com... -
Page 10: Chapter 3 Installation
When it turns red, the pipette needs to be charged. AUTION Use only the approved INTEGRA battery, power supply or charging stand. Use of an incompatible power transformer can damage the pipette. The battery can be charged using either the mains adapter or a charging stand, see (“8.2 Accessories for VIAFLO-Pipettens”... -
Page 11: Attaching And Removing A Viaflo Electronic Pipette
The ASSIST mode is hidden from the Main Menu of the VIAFLO pipettes by default. Use the Touch wheel to select Toolbox from the Main Menu and press Select Preferences and then Main Menu. Enable the ASSIST mode by pressing OK (green ) and press to save your settings. www.integra-biosciences.com... -
Page 12: Overview Of Toolbox For Assist
Sets the tip type standard, short or long (only where applicable). Device Information Contains information about the instrument and the software. Bluetooth Pairing Pairs the INTEGRA pipette and the ASSIST. Move Speed Allows to change the move speed of the pipette arm and the plate carrier. -
Page 13: Adjusting The Plate Alignment
Allows to change the move speed of the pipette arm (1, Z-axis) and the plate carrier (2, X- axis) on ASSIST. Select Move Speed and press OK. Use the Touch wheel to select Slow, Medium or Default (=fast) and press OK. Save your settings. www.integra-biosciences.com... -
Page 14: Chapter 4 Operation
It is possible to create programs, however without teaching of active heights. Running a program From the ASSIST menu select the program category and the stored program you want to run and press OK. Press the Run key (22). INTEGRA Biosciences AG... -
Page 15: Pipetting In 96 And 384 Well Plates
(a) moves the pipette center to pipette in 96 well lever (a) moves the pipette to the rear to accommo- plates. to the front to accommo- date the rear positions of date the front positions of 384 well plates. 384 well plates. www.integra-biosciences.com... -
Page 16: Troubleshooting
“3.6.6 Adjusting the plate tion. alignment” on page 13. The tip height is not • Wrong tip type set. • Enter the tip type used, see correct using 12.5 μl “3.6.5 Setting tip type” on GripTips. page 12. INTEGRA Biosciences AG... -
Page 17: Chapter 5 Programming
Variable Dispense Allows dispensing multiple aliquots of different volumes. Multi Aspirate Allows aspirating multiple aliquots of the same or different volumes from a microplate. Custom Programs Allows to create and store multi-stepped user-defined pipetting protocols. www.integra-biosciences.com... -
Page 18: Setting Up A Program
Modify existing programs At any program display, use the Touch wheel to highlight an existing program. Press Options, use the Touch wheel to select an option (View/Edit, Delete, Copy, Rename) to modify the program and press OK. INTEGRA Biosciences AG... -
Page 19: Adjust Heights And Positions
GripTips at the desired height and press OK to save current settings. Keep the Arrow keys (, , , ) pressed, if you want to increase the movement speed during position teaching. Press the Arrow keys shortly for fine adjustment. www.integra-biosciences.com... -
Page 20: Detailed Description Of Program Categories
Asp Speed Sets speed uniquely for aspiration (1 = low, 10 = fast). Mixing Mixes the sample in the source container before the first aspiration. • If activated, define Mix volume, Mix Speed and number of Mix Cycles. INTEGRA Biosciences AG... - Page 21 • Target Mix: moves the set distance down and up during mixing in the target location. Press to save your settings. This will return you to the list of Serial Dilution programs. www.integra-biosciences.com...
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Page 22: Repeat/Variable Dispense Programs
Set the: • Plate count: the total number of target plates (1-9) used in the current run • Reservoir type, only INTEGRA reservoirs, see 8.3. • Start volume: the filling volume that is currently in the INTEGRA reagent reservoir. - Page 23 50 µl: 2 µl (= 4%), 125 µl: 5 µl (= 4%), 300 µl: 15 µl (= 5%), 1250 µl: 50 µl (= 4%). Press to save your settings. This will return you to the list of Repeat/Variable Dispense programs. www.integra-biosciences.com...
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Page 24: Multi Aspirate Program
• Source Mix: moves the set distance down and up during mixing in the source location. Press to save your settings. This will return you to the list of Multi Aspirate programs. INTEGRA Biosciences AG... -
Page 25: Custom Programs
The distance from well to well for a 96 well plate is 9 mm and for a 384 well plate 4.5 mm. 1. Tip Travel distance: the distance the pipette automatically follows during aspirat- ing, dispensing or mixing allowing to optimize tip immersion depth, see 7.8. www.integra-biosciences.com... - Page 26 A custom program can have a maximum of 10 Call steps. Beep Sets a beep. The sound is only active, if under Preferences - Sounds the option Messages is set to On. When finished, press to save the Custom program. To run the program, press OK. INTEGRA Biosciences AG...
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Page 27: Chapter 6 Maintenance
This is necessary to protect service personnel. Equipment disposal The ASSIST must not be disposed of with unsorted municipal waste. Dispose the ASSIST in accordance with the laws and regulations in your area governing disposal of devices. www.integra-biosciences.com... -
Page 28: Technical Data
10 kg Material of surfaces Polyurethane (Housing) Stainless steel (painted or blank) Aluminium Intellectual Property The ASSIST is covered under the following patents: Patent Number Country Title Apply to 9,321,048 Sample Distribution System And Pro- ASSIST cess INTEGRA Biosciences AG... -
Page 29: Compatible Pipettes
ASSIST – Operating instructions V09 Technical Data Compatible pipettes The following INTEGRA pipettes are compatible with ASSIST: Pipette Firmware Serial No. VIAFLO 3.xx 6xxxxxx VIAFLO 4.xx 7xxxxxx Maximum labware height on plate carrier Maximum distance between piptte tip end and plate carrier. -
Page 30: Tip Travel Distances
) plus Target set Target Disp. (T Height (H ) plus Target Disp. (T ) distance. distance up. Disp. (T ). Moves the Moves the Target Target Mix (T Disp. (T ) distance distance down and up. down. INTEGRA Biosciences AG... -
Page 31: Chapter 8 Accessories
Linear stand, holds up to 4 charging stations 3215 Mains Adapter for linear stand and carousel charging stand 3216 Charging station for linear stand, incl. connection cable 3217 Charging/communication station for linear stand, incl. connection cable 3218 and USB cable Bluetooth module for VIAFLO pipettes 4221 www.integra-biosciences.com... -
Page 32: Consumables
30 reservoirs per case, sterile Disposable reagent reservoirs, four sleeves of 50 reservoirs, 4322 200 reservoirs per case, sterile Reservoir Base, 10 pack 4305 GripTips for all INTEGRA Pipettes Part No. Purple 5 XYZ racks of 384 tips, sterile, filter, SHORT 6475 SHORT: 12.5 μl... - Page 33 ASSIST – Operating instructions V09 Accessories GripTips for all INTEGRA Pipettes Part No. Purple: Bulk pack, 1 bag of 1000 tips, non-sterile 4411 12.5 μl 5 inserts of 384 tips, non-sterile, GREEN CHOICE 4412 5 racks of 384 tips, non-sterile...
- Page 34 ASSIST – Operating instructions V09 Accessories GripTips for all INTEGRA Pipettes Part No. Blue: Bulk pack, 1 bag of 500 tips, non-sterile 4441 1250 μl 5 inserts of 96 tips, non-sterile, GREEN CHOICE 4442 5 racks of 96 Tips, non-sterile...
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Page 35: Imprint
© 2018 INTEGRA Biosciences AG All rights to this documentation are reserved. In particular the rights of reproduction, processing, translation and the form of presentation lie with INTEGRA Biosciences AG. Neither the complete documentation nor parts thereof may be reproduced in any way, or stored and processed using electronic media or distributed in any other way without the written consent of INTEGRA Biosciences AG.
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